Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 12920 and 12921 are separated by 948 nucleotides12921 and 12922 are separated by 122 nucleotides12922 and 12923 are separated by 639 nucleotides12923 and 12924 are separated by 163 nucleotides mRNA_4552: 12920 - KOG2263 Methionine synthase II (cobalamin-independent), at 442,717 to 444,581 12920 mRNA_4553: 12921 - SUPERFAMILY--SSF81383, at 445,530 to 447,635 12921 mRNA_4554: 12922 - K12585 DIS3, RRP44 exosome complex exonuclease DIS3/RRP44, at 447,758 to 451,562 12922 mRNA_4555: 12923 - KOG3855 Monooxygenase involved in coenzyme Q (ubiquinone) biosynthesis, at 452,202 to 455,003 12923 mRNA_4556: 12924 - KOG1294 Apurinic/apyrimidinic endonuclease and related enzymes, at 455,167 to 456,572 12924
Group Condition mRNA_4552 mRNA_4553 mRNA_4554 mRNA_4555 mRNA_4556
12920 12921 12922 12923 12924
auxotrophy YNB Glucose 75mM Methionine -0.1 -0.1 N.D. -0.0 -2.1
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation -0.5 -0.3 N.D. -0.5 -0.7
auxotrophy Dropout Complete -0.3 -0.3 N.D. -0.1 -0.8
buoyancy on lipid accumulation 40 hrs Low Buoyancy -0.1 -0.5 N.D. -0.2 -0.5
auxotrophy YNB Glucose 75mM Arginine -0.2 -0.1 N.D. -0.1 -0.8
buoyancy on lipid accumulation 40 hrs High Buoyancy -0.4 -0.1 N.D. -0.1 -0.6
auxotrophy YNB Glucose -0.6 +0.1 N.D. -0.1 -0.3
growth on ricinoleic acid YNB Glucose -0.5 +0.1 N.D. -0.3 -0.2
growth on ricinoleic acid YNB Ricinoleic Acid +0.1 +0.4 N.D. -0.6 -0.8
facs on lipid accumulation Sorted High BODIPY Gate +0.2 -0.0 N.D. +0.2 -1.2
buoyancy on lipid accumulation 40 hrs Median Buoyancy +0.0 -0.0 N.D. -0.1 -0.5
facs on lipid accumulation Sorted No BODIPY Gate -0.4 +0.0 N.D. +0.2 -0.4
growth on oleic acid YNB Glucose +0.2 +0.0 N.D. -0.4 -0.3
growth on ricinoleic acid YNB Methylricinoleic Acid -0.0 +0.3 N.D. +0.1 -0.8
facs on lipid accumulation Sorted Low BODIPY Gate -0.1 +0.0 N.D. +0.4 -0.7
ypd buoyancy 40 hrs High Buoyancy -0.0 +0.2 N.D. -0.2 -0.3
ypd buoyancy 40 hrs Low Buoyancy -0.0 +0.2 N.D. -0.1 -0.2
growth on ypd YPD +0.1 +0.3 N.D. +0.3 -0.8
growth on oleic acid YNB Oleic Acid +0.2 +0.1 N.D. +0.2 -0.1
ypd buoyancy 40 hrs Median Buoyancy -0.0 +0.2 N.D. +0.3 +0.0
ypd buoyancy 40 hrs No Buoyancy Separation -0.0 +0.3 N.D. -0.0 +0.5
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