Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 12925 and 12926 are separated by 233 nucleotides12926 and 12927 are separated by 974 nucleotides12927 and 12928 are separated by 558 nucleotides12928 and 12929 are separated by 208 nucleotides mRNA_4557: 12925 - KOG3318 Predicted membrane protein, at 456,635 to 457,680 12925 mRNA_4558: 12926 - K03320 amt, AMT, MEP ammonium transporter, Amt family, at 457,914 to 459,915 12926 mRNA_4559: 12927 - HMMPfam-PAP2 superfamily-PF01569,SMART-Acid phosphatase homologues-SM00014,SUPERFAMILY--SSF48317, at 460,890 to 462,526 12927 mRNA_4560: 12928 - Hypothetical Protein, at 463,085 to 464,279 12928 mRNA_4561: 12929 - K06990 MEMO1 MEMO1 family protein, at 464,488 to 466,003 12929
Group Condition mRNA_4557 mRNA_4558 mRNA_4559 mRNA_4560 mRNA_4561
12925 12926 12927 12928 12929
facs on lipid accumulation Sorted High BODIPY Gate +0.1 -0.5 N.D. -1.3 -1.5
facs on lipid accumulation Sorted Low BODIPY Gate +0.3 -1.1 N.D. -0.3 -1.0
facs on lipid accumulation Sorted No BODIPY Gate +0.2 -0.6 N.D. -0.5 -1.2
buoyancy on lipid accumulation 40 hrs High Buoyancy -0.7 -0.5 N.D. +0.4 -1.0
auxotrophy YNB Glucose 75mM Arginine +0.1 -0.4 N.D. -0.5 -0.2
growth on ricinoleic acid YNB Methylricinoleic Acid +0.2 -0.5 N.D. -0.4 -0.1
ypd buoyancy 40 hrs No Buoyancy Separation -0.2 -0.1 N.D. -0.1 -0.4
auxotrophy YNB Glucose 75mM Methionine +0.3 -0.3 N.D. -0.5 -0.0
growth on oleic acid YNB Oleic Acid -0.1 -0.4 N.D. +0.1 +0.2
growth on ricinoleic acid YNB Ricinoleic Acid -0.0 +0.2 N.D. -0.6 +0.2
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation -0.5 -0.1 N.D. +0.4 +0.1
growth on oleic acid YNB Glucose -0.1 -0.2 N.D. -0.1 +0.4
buoyancy on lipid accumulation 40 hrs Low Buoyancy -0.3 -0.3 N.D. +0.3 +0.3
ypd buoyancy 40 hrs Low Buoyancy -0.0 -0.5 N.D. +0.4 +0.0
auxotrophy Dropout Complete +0.0 +0.0 N.D. -0.2 +0.3
growth on ricinoleic acid YNB Glucose +0.2 -0.1 N.D. +0.0 -0.1
buoyancy on lipid accumulation 40 hrs Median Buoyancy -0.2 -0.1 N.D. +0.9 -0.4
ypd buoyancy 40 hrs Median Buoyancy -0.0 -0.1 N.D. +0.1 +0.3
auxotrophy YNB Glucose +0.2 -0.1 N.D. +0.1 +0.1
ypd buoyancy 40 hrs High Buoyancy +0.3 -0.4 N.D. +0.1 +0.5
growth on ypd YPD -0.1 +0.2 N.D. -0.0 +0.7
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