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Fitness for 5 genes in
Rhodosporidium toruloides IFO0880
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Top 21 experiments (either direction), sorted by average fitness
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500 nt
12930 and 12931 are separated by 551 nucleotides
12931 and 12932 are separated by 675 nucleotides
12932 and 12933 are separated by 478 nucleotides
12933 and 12934 are separated by 147 nucleotides
mRNA_4562: 12930 - K11366 USP22_27_51, UBP8 ubiquitin carboxyl-terminal hydrolase 22/27/51, at 466,499 to 468,748
12930
mRNA_4563: 12931 - KOG1601 GATA-4/5/6 transcription factors, at 469,300 to 472,637
12931
mRNA_4564: 12932 - K11844 USP16_45 ubiquitin carboxyl-terminal hydrolase 16/45, at 473,313 to 477,203
12932
mRNA_4565: 12933 - KOG2793 Putative N2,N2-dimethylguanosine tRNA methyltransferase, at 477,682 to 479,259
12933
mRNA_4566: 12934 - KOG2273 Membrane coat complex Retromer, subunit VPS5/SNX1, Sorting nexins, and related PX domain-containing proteins, at 479,407 to 483,783
12934
Group
Condition
mRNA
_4562
mRNA
_4563
mRNA
_4564
mRNA
_4565
mRNA
_4566
12930
12931
12932
12933
12934
buoyancy on lipid accumulation
40 hrs Low Buoyancy
+0.1
-0.6
-1.0
-0.5
-0.0
growth on ricinoleic acid
YNB Ricinoleic Acid
-0.4
-0.0
+0.1
-1.0
-0.1
auxotrophy
Dropout Complete
-0.6
-0.7
-0.2
+0.1
+0.4
growth on ricinoleic acid
YNB Glucose
-0.3
+0.4
-0.3
-0.7
-0.1
buoyancy on lipid accumulation
40 hrs No Buoyancy Separation
-0.2
-0.6
+0.1
-0.0
-0.2
auxotrophy
YNB Glucose 75mM Methionine
-0.4
-0.6
+0.1
-0.1
+0.2
auxotrophy
YNB Glucose 75mM Arginine
-0.4
-0.4
-0.1
-0.0
+0.2
auxotrophy
YNB Glucose
-0.5
-0.2
-0.3
-0.1
+0.3
ypd buoyancy
40 hrs No Buoyancy Separation
+0.8
-0.0
-0.3
-0.5
-0.5
facs on lipid accumulation
Sorted No BODIPY Gate
-0.1
-0.5
+0.7
-0.1
-0.1
buoyancy on lipid accumulation
40 hrs Median Buoyancy
-0.1
-0.2
+0.6
-0.2
-0.2
ypd buoyancy
40 hrs Low Buoyancy
-0.0
-0.1
-0.3
+0.4
+0.1
growth on oleic acid
YNB Glucose
-0.3
+0.2
+0.3
-0.1
-0.0
growth on ypd
YPD
-0.1
+0.0
+0.2
-0.0
+0.1
facs on lipid accumulation
Sorted High BODIPY Gate
-0.4
+1.0
+0.0
-0.3
-0.1
growth on ricinoleic acid
YNB Methylricinoleic Acid
-0.1
+0.4
+0.3
-0.2
+0.0
growth on oleic acid
YNB Oleic Acid
-0.2
-0.3
+1.1
+0.1
-0.1
buoyancy on lipid accumulation
40 hrs High Buoyancy
-0.2
-0.1
+1.0
-0.1
-0.1
facs on lipid accumulation
Sorted Low BODIPY Gate
+0.2
-0.5
+1.4
+0.3
-0.5
ypd buoyancy
40 hrs High Buoyancy
+0.2
+0.2
+0.4
+0.3
+0.1
ypd buoyancy
40 hrs Median Buoyancy
-0.1
+0.8
+0.4
+0.4
+0.1
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