Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 12933 and 12934 are separated by 147 nucleotides12934 and 12935 are separated by 268 nucleotides12935 and 12936 are separated by 219 nucleotides12936 and 12937 are separated by 155 nucleotides mRNA_4565: 12933 - KOG2793 Putative N2,N2-dimethylguanosine tRNA methyltransferase, at 477,682 to 479,259 12933 mRNA_4566: 12934 - KOG2273 Membrane coat complex Retromer, subunit VPS5/SNX1, Sorting nexins, and related PX domain-containing proteins, at 479,407 to 483,783 12934 mRNA_4567: 12935 - K10743 RNASEH2A ribonuclease H2 subunit A, at 484,052 to 485,585 12935 mRNA_4568: 12936 - Hypothetical Protein, at 485,805 to 489,325 12936 mRNA_4569: 12937 - HMMPfam-Integral peroxisomal membrane peroxin-PF06398, at 489,481 to 490,955 12937
Group Condition mRNA_4565 mRNA_4566 mRNA_4567 mRNA_4568 mRNA_4569
12933 12934 12935 12936 12937
growth on oleic acid YNB Glucose -0.1 -0.0 -0.1 -0.1 -1.2
facs on lipid accumulation Sorted Low BODIPY Gate +0.3 -0.5 -0.7 +0.1 -0.7
growth on oleic acid YNB Oleic Acid +0.1 -0.1 +0.1 -0.1 -0.7
growth on ricinoleic acid YNB Glucose -0.7 -0.1 -0.0 -0.3 +0.5
facs on lipid accumulation Sorted No BODIPY Gate -0.1 -0.1 -0.4 +0.1 +0.0
buoyancy on lipid accumulation 40 hrs Low Buoyancy -0.5 -0.0 -0.0 -0.1 +0.3
growth on ricinoleic acid YNB Ricinoleic Acid -1.0 -0.1 +0.4 -0.0 +0.2
growth on ricinoleic acid YNB Methylricinoleic Acid -0.2 +0.0 +0.1 -0.1 -0.1
ypd buoyancy 40 hrs Low Buoyancy +0.4 +0.1 +0.2 -0.1 -0.9
ypd buoyancy 40 hrs No Buoyancy Separation -0.5 -0.5 +0.7 -0.3 +0.4
facs on lipid accumulation Sorted High BODIPY Gate -0.3 -0.1 -0.3 -0.1 +0.8
auxotrophy YNB Glucose 75mM Methionine -0.1 +0.2 +0.1 -0.2 -0.0
ypd buoyancy 40 hrs Median Buoyancy +0.4 +0.1 +0.3 -0.2 -0.5
ypd buoyancy 40 hrs High Buoyancy +0.3 +0.1 +0.2 -0.2 +0.0
growth on ypd YPD -0.0 +0.1 +0.1 +0.0 +0.4
buoyancy on lipid accumulation 40 hrs Median Buoyancy -0.2 -0.2 +0.1 +0.2 +1.1
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation -0.0 -0.2 -0.3 -0.1 +1.7
auxotrophy Dropout Complete +0.1 +0.4 +0.1 -0.3 +0.9
auxotrophy YNB Glucose -0.1 +0.3 +0.4 -0.2 +0.6
buoyancy on lipid accumulation 40 hrs High Buoyancy -0.1 -0.1 +0.3 +0.1 +1.0
auxotrophy YNB Glucose 75mM Arginine -0.0 +0.2 +0.4 -0.1 +1.0
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