Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 12940 and 12941 are separated by 123 nucleotides12941 and 12942 are separated by 54 nucleotides12942 and 12943 are separated by 271 nucleotides12943 and 12944 are separated by 287 nucleotides mRNA_4572: 12940 - K06963 TAN1, THUMPD1 tRNA acetyltransferase TAN1, at 495,766 to 497,109 12940 mRNA_4573: 12941 - K03142 TFIIH2, GTF2H2, SSL1 transcription initiation factor TFIIH subunit 2, at 497,233 to 498,989 12941 mRNA_4574: 12942 - K12184 VPS28 ESCRT-I complex subunit VPS28, at 499,044 to 500,144 12942 mRNA_4575: 12943 - HMMPfam-ICE2-PF08426, at 500,416 to 502,164 12943 mRNA_4576: 12944 - K00787 FDPS farnesyl diphosphate synthase, at 502,452 to 503,977 12944
Group Condition mRNA_4572 mRNA_4573 mRNA_4574 mRNA_4575 mRNA_4576
12940 12941 12942 12943 12944
facs on lipid accumulation Sorted High BODIPY Gate N.D. N.D. N.D. -0.6 N.D.
buoyancy on lipid accumulation 40 hrs Median Buoyancy N.D. N.D. N.D. -0.4 N.D.
facs on lipid accumulation Sorted No BODIPY Gate N.D. N.D. N.D. -0.3 N.D.
facs on lipid accumulation Sorted Low BODIPY Gate N.D. N.D. N.D. -0.2 N.D.
buoyancy on lipid accumulation 40 hrs High Buoyancy N.D. N.D. N.D. -0.2 N.D.
growth on oleic acid YNB Oleic Acid N.D. N.D. N.D. -0.1 N.D.
growth on ypd YPD N.D. N.D. N.D. -0.1 N.D.
auxotrophy YNB Glucose 75mM Arginine N.D. N.D. N.D. -0.1 N.D.
auxotrophy Dropout Complete N.D. N.D. N.D. -0.1 N.D.
ypd buoyancy 40 hrs Median Buoyancy N.D. N.D. N.D. -0.1 N.D.
buoyancy on lipid accumulation 40 hrs Low Buoyancy N.D. N.D. N.D. -0.1 N.D.
auxotrophy YNB Glucose 75mM Methionine N.D. N.D. N.D. -0.1 N.D.
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation N.D. N.D. N.D. -0.0 N.D.
ypd buoyancy 40 hrs Low Buoyancy N.D. N.D. N.D. +0.1 N.D.
ypd buoyancy 40 hrs High Buoyancy N.D. N.D. N.D. +0.2 N.D.
auxotrophy YNB Glucose N.D. N.D. N.D. +0.2 N.D.
growth on oleic acid YNB Glucose N.D. N.D. N.D. +0.3 N.D.
ypd buoyancy 40 hrs No Buoyancy Separation N.D. N.D. N.D. +0.5 N.D.
growth on ricinoleic acid YNB Methylricinoleic Acid N.D. N.D. N.D. +0.7 N.D.
growth on ricinoleic acid YNB Ricinoleic Acid N.D. N.D. N.D. +0.8 N.D.
growth on ricinoleic acid YNB Glucose N.D. N.D. N.D. +0.9 N.D.
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