Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 12947 and 12948 are separated by 1220 nucleotides12948 and 12949 are separated by 265 nucleotides12949 and 12950 are separated by 497 nucleotides12950 and 12951 are separated by 459 nucleotides mRNA_4579: 12947 - KOG1399 Flavin-containing monooxygenase, at 508,671 to 511,163 12947 mRNA_4580: 12948 - Hypothetical Protein, at 512,384 to 513,483 12948 mRNA_4581: 12949 - K17964 LRPPRC leucine-rich PPR motif-containing protein, mitochondrial, at 513,749 to 516,613 12949 mRNA_4582: 12950 - KOG2450 Aldehyde dehydrogenase, at 517,111 to 519,102 12950 mRNA_4583: 12951 - Hypothetical Protein, at 519,562 to 522,262 12951
Group Condition mRNA_4579 mRNA_4580 mRNA_4581 mRNA_4582 mRNA_4583
12947 12948 12949 12950 12951
growth on ricinoleic acid YNB Ricinoleic Acid -0.5 -0.2 N.D. +0.1 -1.0
buoyancy on lipid accumulation 40 hrs Median Buoyancy -1.3 -0.3 N.D. +0.0 -0.0
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation -0.3 -0.3 N.D. -0.1 -0.7
buoyancy on lipid accumulation 40 hrs High Buoyancy -0.3 -0.2 N.D. -0.2 -0.6
growth on ricinoleic acid YNB Methylricinoleic Acid -0.1 -0.2 N.D. -0.0 -0.8
ypd buoyancy 40 hrs Low Buoyancy -0.2 -0.1 N.D. -0.0 -0.2
ypd buoyancy 40 hrs High Buoyancy -0.2 -0.1 N.D. -0.1 -0.3
growth on oleic acid YNB Glucose +0.0 -0.0 N.D. -0.2 -0.4
growth on oleic acid YNB Oleic Acid -0.3 +0.2 N.D. +0.0 -0.4
growth on ricinoleic acid YNB Glucose +0.3 +0.0 N.D. -0.1 -0.5
facs on lipid accumulation Sorted Low BODIPY Gate -0.2 +0.5 N.D. -0.4 -0.2
facs on lipid accumulation Sorted High BODIPY Gate -0.1 -0.2 N.D. -0.0 +0.1
growth on ypd YPD +0.2 +0.1 N.D. +0.2 -0.7
ypd buoyancy 40 hrs No Buoyancy Separation -0.6 -0.2 N.D. +0.7 +0.1
buoyancy on lipid accumulation 40 hrs Low Buoyancy +0.5 +0.1 N.D. -0.2 -0.4
ypd buoyancy 40 hrs Median Buoyancy -0.2 +0.2 N.D. +0.1 +0.1
auxotrophy YNB Glucose +0.1 +0.1 N.D. +0.2 -0.2
auxotrophy YNB Glucose 75mM Methionine +0.1 +0.2 N.D. +0.2 -0.0
facs on lipid accumulation Sorted No BODIPY Gate +0.4 +0.6 N.D. +0.1 -0.3
auxotrophy YNB Glucose 75mM Arginine +0.2 +0.7 N.D. +0.2 -0.3
auxotrophy Dropout Complete -0.0 +0.7 N.D. +0.3 -0.2
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