Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 12960 and 12961 are separated by 820 nucleotides12961 and 12962 are separated by 114 nucleotides12962 and 12963 are separated by 556 nucleotides12963 and 12964 are separated by 244 nucleotides mRNA_4592: 12960 - K11655 BAZ1A, ACF1 bromodomain adjacent to zinc finger domain protein 1A, at 541,515 to 545,296 12960 mRNA_4593: 12961 - K05286 PIGB phosphatidylinositol glycan, class B, at 546,117 to 548,213 12961 mRNA_4594: 12962 - K19589 N6AMT1 release factor glutamine methyltransferase, at 548,328 to 549,291 12962 mRNA_4595: 12963 - KOG2186 Cell growth-regulating nucleolar protein, at 549,848 to 550,552 12963 mRNA_4596: 12964 - KOG4009 NADH-ubiquinone oxidoreductase, subunit NDUFB10/PDSW, at 550,797 to 551,736 12964
Group Condition mRNA_4592 mRNA_4593 mRNA_4594 mRNA_4595 mRNA_4596
12960 12961 12962 12963 12964
facs on lipid accumulation Sorted No BODIPY Gate -1.7 N.D. N.D. -1.5 N.D.
facs on lipid accumulation Sorted High BODIPY Gate -1.9 N.D. N.D. -0.1 N.D.
ypd buoyancy 40 hrs Low Buoyancy -0.8 N.D. N.D. -1.3 N.D.
facs on lipid accumulation Sorted Low BODIPY Gate -2.4 N.D. N.D. +0.5 N.D.
auxotrophy Dropout Complete -2.4 N.D. N.D. +0.8 N.D.
auxotrophy YNB Glucose -2.1 N.D. N.D. +0.7 N.D.
ypd buoyancy 40 hrs Median Buoyancy -0.5 N.D. N.D. -0.3 N.D.
ypd buoyancy 40 hrs No Buoyancy Separation -0.8 N.D. N.D. +0.0 N.D.
auxotrophy YNB Glucose 75mM Methionine -1.1 N.D. N.D. +0.7 N.D.
auxotrophy YNB Glucose 75mM Arginine -0.9 N.D. N.D. +0.6 N.D.
growth on oleic acid YNB Glucose -0.2 N.D. N.D. +0.0 N.D.
growth on ricinoleic acid YNB Glucose -2.0 N.D. N.D. +1.9 N.D.
growth on ypd YPD -0.4 N.D. N.D. +0.3 N.D.
buoyancy on lipid accumulation 40 hrs High Buoyancy +1.7 N.D. N.D. -1.7 N.D.
ypd buoyancy 40 hrs High Buoyancy +1.8 N.D. N.D. -1.4 N.D.
growth on ricinoleic acid YNB Ricinoleic Acid -0.2 N.D. N.D. +1.1 N.D.
growth on oleic acid YNB Oleic Acid +1.3 N.D. N.D. -0.2 N.D.
growth on ricinoleic acid YNB Methylricinoleic Acid -0.1 N.D. N.D. +1.1 N.D.
buoyancy on lipid accumulation 40 hrs Low Buoyancy +1.0 N.D. N.D. +0.2 N.D.
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation +1.5 N.D. N.D. +0.3 N.D.
buoyancy on lipid accumulation 40 hrs Median Buoyancy +1.8 N.D. N.D. +0.1 N.D.
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