Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 12964 and 12965 are separated by 101 nucleotides12965 and 12966 are separated by 581 nucleotides12966 and 12967 are separated by 77 nucleotides12967 and 12968 are separated by 97 nucleotides mRNA_4596: 12964 - KOG4009 NADH-ubiquinone oxidoreductase, subunit NDUFB10/PDSW, at 550,797 to 551,736 12964 mRNA_4597: 12965 - K01836 PGM3 phosphoacetylglucosamine mutase, at 551,838 to 553,839 12965 mRNA_4598: 12966 - K00418 QCR8, UQCRQ ubiquinol-cytochrome c reductase subunit 8, at 554,421 to 554,905 12966 mRNA_4599: 12967 - K12820 DHX15, PRP43 pre-mRNA-splicing factor ATP-dependent RNA helicase DHX15/PRP43, at 554,983 to 558,487 12967 mRNA_4600: 12968 - K08343 ATG3 ubiquitin-like-conjugating enzyme ATG3, at 558,585 to 560,759 12968
Group Condition mRNA_4596 mRNA_4597 mRNA_4598 mRNA_4599 mRNA_4600
12964 12965 12966 12967 12968
facs on lipid accumulation Sorted High BODIPY Gate N.D. N.D. N.D. N.D. -4.3
facs on lipid accumulation Sorted No BODIPY Gate N.D. N.D. N.D. N.D. -1.8
buoyancy on lipid accumulation 40 hrs Median Buoyancy N.D. N.D. N.D. N.D. -1.8
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation N.D. N.D. N.D. N.D. -0.6
growth on ricinoleic acid YNB Ricinoleic Acid N.D. N.D. N.D. N.D. -0.5
auxotrophy YNB Glucose 75mM Methionine N.D. N.D. N.D. N.D. -0.5
auxotrophy YNB Glucose N.D. N.D. N.D. N.D. -0.4
growth on ricinoleic acid YNB Methylricinoleic Acid N.D. N.D. N.D. N.D. -0.3
auxotrophy YNB Glucose 75mM Arginine N.D. N.D. N.D. N.D. -0.3
ypd buoyancy 40 hrs No Buoyancy Separation N.D. N.D. N.D. N.D. -0.3
buoyancy on lipid accumulation 40 hrs High Buoyancy N.D. N.D. N.D. N.D. -0.2
ypd buoyancy 40 hrs Low Buoyancy N.D. N.D. N.D. N.D. -0.2
ypd buoyancy 40 hrs Median Buoyancy N.D. N.D. N.D. N.D. -0.1
growth on oleic acid YNB Oleic Acid N.D. N.D. N.D. N.D. -0.1
growth on ricinoleic acid YNB Glucose N.D. N.D. N.D. N.D. -0.0
ypd buoyancy 40 hrs High Buoyancy N.D. N.D. N.D. N.D. -0.0
auxotrophy Dropout Complete N.D. N.D. N.D. N.D. +0.0
growth on oleic acid YNB Glucose N.D. N.D. N.D. N.D. +0.1
facs on lipid accumulation Sorted Low BODIPY Gate N.D. N.D. N.D. N.D. +0.3
growth on ypd YPD N.D. N.D. N.D. N.D. +0.6
buoyancy on lipid accumulation 40 hrs Low Buoyancy N.D. N.D. N.D. N.D. +0.6
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