Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 12966 and 12967 are separated by 77 nucleotides12967 and 12968 are separated by 97 nucleotides12968 and 12969 are separated by 115 nucleotides12969 and 12970 are separated by 285 nucleotides mRNA_4598: 12966 - K00418 QCR8, UQCRQ ubiquinol-cytochrome c reductase subunit 8, at 554,421 to 554,905 12966 mRNA_4599: 12967 - K12820 DHX15, PRP43 pre-mRNA-splicing factor ATP-dependent RNA helicase DHX15/PRP43, at 554,983 to 558,487 12967 mRNA_4600: 12968 - K08343 ATG3 ubiquitin-like-conjugating enzyme ATG3, at 558,585 to 560,759 12968 mRNA_4601: 12969 - HMMPfam-Sen15 protein-PF09631,SUPERFAMILY--SSF53032, at 560,875 to 561,580 12969 mRNA_4602: 12970 - HMMPfam-NYN domain-PF01936, at 561,866 to 562,949 12970
Group Condition mRNA_4598 mRNA_4599 mRNA_4600 mRNA_4601 mRNA_4602
12966 12967 12968 12969 12970
facs on lipid accumulation Sorted High BODIPY Gate N.D. N.D. -4.3 N.D. -0.1
facs on lipid accumulation Sorted No BODIPY Gate N.D. N.D. -1.8 N.D. -0.2
buoyancy on lipid accumulation 40 hrs Median Buoyancy N.D. N.D. -1.8 N.D. +0.2
growth on ricinoleic acid YNB Ricinoleic Acid N.D. N.D. -0.5 N.D. -0.4
ypd buoyancy 40 hrs No Buoyancy Separation N.D. N.D. -0.3 N.D. -0.6
growth on ricinoleic acid YNB Methylricinoleic Acid N.D. N.D. -0.3 N.D. -0.3
growth on oleic acid YNB Glucose N.D. N.D. +0.1 N.D. -0.6
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation N.D. N.D. -0.6 N.D. +0.2
auxotrophy YNB Glucose 75mM Methionine N.D. N.D. -0.5 N.D. +0.2
growth on oleic acid YNB Oleic Acid N.D. N.D. -0.1 N.D. -0.2
growth on ricinoleic acid YNB Glucose N.D. N.D. -0.0 N.D. -0.2
ypd buoyancy 40 hrs Median Buoyancy N.D. N.D. -0.1 N.D. -0.1
auxotrophy YNB Glucose N.D. N.D. -0.4 N.D. +0.2
auxotrophy YNB Glucose 75mM Arginine N.D. N.D. -0.3 N.D. +0.2
ypd buoyancy 40 hrs Low Buoyancy N.D. N.D. -0.2 N.D. +0.2
ypd buoyancy 40 hrs High Buoyancy N.D. N.D. -0.0 N.D. -0.0
buoyancy on lipid accumulation 40 hrs High Buoyancy N.D. N.D. -0.2 N.D. +0.3
auxotrophy Dropout Complete N.D. N.D. +0.0 N.D. +0.1
facs on lipid accumulation Sorted Low BODIPY Gate N.D. N.D. +0.3 N.D. -0.1
buoyancy on lipid accumulation 40 hrs Low Buoyancy N.D. N.D. +0.6 N.D. -0.1
growth on ypd YPD N.D. N.D. +0.6 N.D. +0.6
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