Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 12972 and 12973 are separated by 238 nucleotides12973 and 12974 are separated by 261 nucleotides12974 and 12975 are separated by 84 nucleotides12975 and 12976 are separated by 591 nucleotides mRNA_4604: 12972 - K10747 LIG1 DNA ligase 1, at 565,709 to 567,987 12972 mRNA_4605: 12973 - KOG0254 Predicted transporter (major facilitator superfamily), at 568,226 to 569,633 12973 mRNA_4606: 12974 - HMMPfam-Zinc-binding dehydrogenase-PF00107,HMMPfam-Alcohol dehydrogenase GroES-like domain-PF08240,ProSitePatterns-Zinc-containing alcohol dehydrogenases signature.-PS00059,SUPERFAMILY--SSF50129,SUPERFAMILY--SSF51735, at 569,895 to 571,183 12974 mRNA_4607: 12975 - BLAST TrfA domain protein, putative [Rhizoctonia solani AG-..., at 571,268 to 571,973 12975 mRNA_4608: 12976 - KOG0254 Predicted transporter (major facilitator superfamily), at 572,565 to 575,380 12976
Group Condition mRNA_4604 mRNA_4605 mRNA_4606 mRNA_4607 mRNA_4608
12972 12973 12974 12975 12976
facs on lipid accumulation Sorted Low BODIPY Gate N.D. +0.1 +0.1 -0.4 -0.6
growth on oleic acid YNB Glucose N.D. +0.4 +0.3 -1.3 -0.0
growth on ypd YPD N.D. +0.4 -0.5 -0.3 -0.2
growth on ricinoleic acid YNB Ricinoleic Acid N.D. +0.2 -0.0 -0.7 -0.0
growth on ricinoleic acid YNB Methylricinoleic Acid N.D. -0.1 +0.1 -0.6 +0.2
facs on lipid accumulation Sorted High BODIPY Gate N.D. -0.4 +0.1 +0.1 -0.2
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation N.D. +0.7 +0.2 -0.7 -0.5
facs on lipid accumulation Sorted No BODIPY Gate N.D. +0.1 +0.5 -0.4 -0.4
growth on oleic acid YNB Oleic Acid N.D. -0.0 +0.1 -0.6 +0.3
auxotrophy YNB Glucose 75mM Arginine N.D. +0.8 -0.0 -1.1 +0.3
auxotrophy YNB Glucose 75mM Methionine N.D. +1.0 +0.2 -0.9 -0.2
buoyancy on lipid accumulation 40 hrs High Buoyancy N.D. +0.4 +0.4 -0.7 +0.0
auxotrophy Dropout Complete N.D. +0.8 +0.2 -0.8 +0.1
auxotrophy YNB Glucose N.D. +0.9 +0.3 -0.7 +0.1
ypd buoyancy 40 hrs Median Buoyancy N.D. +0.1 +0.2 +0.1 +0.2
ypd buoyancy 40 hrs Low Buoyancy N.D. +0.4 -0.2 +0.6 -0.1
ypd buoyancy 40 hrs High Buoyancy N.D. -0.2 +0.2 +0.3 +0.6
buoyancy on lipid accumulation 40 hrs Low Buoyancy N.D. +0.6 +0.3 -0.1 +0.1
buoyancy on lipid accumulation 40 hrs Median Buoyancy N.D. +0.5 +0.3 -0.2 +0.2
growth on ricinoleic acid YNB Glucose N.D. +0.1 +0.2 +0.1 +0.5
ypd buoyancy 40 hrs No Buoyancy Separation N.D. -0.2 -0.0 +0.9 +0.9
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