Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 12974 and 12975 are separated by 84 nucleotides12975 and 12976 are separated by 591 nucleotides12976 and 12977 are separated by 350 nucleotides12977 and 12978 are separated by 1223 nucleotides mRNA_4606: 12974 - HMMPfam-Zinc-binding dehydrogenase-PF00107,HMMPfam-Alcohol dehydrogenase GroES-like domain-PF08240,ProSitePatterns-Zinc-containing alcohol dehydrogenases signature.-PS00059,SUPERFAMILY--SSF50129,SUPERFAMILY--SSF51735, at 569,895 to 571,183 12974 mRNA_4607: 12975 - BLAST TrfA domain protein, putative [Rhizoctonia solani AG-..., at 571,268 to 571,973 12975 mRNA_4608: 12976 - KOG0254 Predicted transporter (major facilitator superfamily), at 572,565 to 575,380 12976 mRNA_4609: 12977 - K00008 SORD, gutB L-iditol 2-dehydrogenase, at 575,731 to 577,281 12977 mRNA_4610: 12978 - HMMPfam-Fungal Zn(2)-Cys(6) binuclear cluster domain-PF00172,HMMPfam-Fungal specific transcription factor domain-PF04082,ProSiteProfiles-Zn(2)-C6 fungal-type DNA-binding domain profile.-PS50048,SMART-GAL4-like Zn(II)2Cys6 (or C6 zinc) binuclear cluster DNA-binding domain-SM00066,SMART-Fungal specific transcription factor domain-SM00906,SUPERFAMILY--SSF57701, at 578,505 to 580,784 12978
Group Condition mRNA_4606 mRNA_4607 mRNA_4608 mRNA_4609 mRNA_4610
12974 12975 12976 12977 12978
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation +0.2 -0.7 -0.5 -0.5 +0.0
growth on oleic acid YNB Glucose +0.3 -1.3 -0.0 -0.3 +0.0
facs on lipid accumulation Sorted Low BODIPY Gate +0.1 -0.4 -0.6 -0.0 -0.3
auxotrophy Dropout Complete +0.2 -0.8 +0.1 -0.7 +0.0
growth on ypd YPD -0.5 -0.3 -0.2 -0.2 +0.2
facs on lipid accumulation Sorted No BODIPY Gate +0.5 -0.4 -0.4 -0.2 -0.2
auxotrophy YNB Glucose 75mM Arginine -0.0 -1.1 +0.3 -0.0 +0.2
facs on lipid accumulation Sorted High BODIPY Gate +0.1 +0.1 -0.2 -0.4 -0.3
auxotrophy YNB Glucose +0.3 -0.7 +0.1 -0.4 +0.0
auxotrophy YNB Glucose 75mM Methionine +0.2 -0.9 -0.2 +0.1 +0.1
buoyancy on lipid accumulation 40 hrs High Buoyancy +0.4 -0.7 +0.0 -0.1 -0.3
growth on ricinoleic acid YNB Ricinoleic Acid -0.0 -0.7 -0.0 +0.0 +0.4
buoyancy on lipid accumulation 40 hrs Low Buoyancy +0.3 -0.1 +0.1 -0.3 +0.0
growth on oleic acid YNB Oleic Acid +0.1 -0.6 +0.3 +0.1 +0.1
growth on ricinoleic acid YNB Methylricinoleic Acid +0.1 -0.6 +0.2 -0.0 +0.4
buoyancy on lipid accumulation 40 hrs Median Buoyancy +0.3 -0.2 +0.2 -0.1 -0.1
ypd buoyancy 40 hrs Median Buoyancy +0.2 +0.1 +0.2 -0.2 +0.0
ypd buoyancy 40 hrs Low Buoyancy -0.2 +0.6 -0.1 +0.0 +0.2
ypd buoyancy 40 hrs High Buoyancy +0.2 +0.3 +0.6 -0.3 +0.3
growth on ricinoleic acid YNB Glucose +0.2 +0.1 +0.5 -0.2 +0.3
ypd buoyancy 40 hrs No Buoyancy Separation -0.0 +0.9 +0.9 +0.3 -0.7
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