Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 12978 and 12979 are separated by 325 nucleotides12979 and 12980 are separated by 6200 nucleotides12980 and 12981 are separated by 3557 nucleotides12981 and 12982 are separated by 1049 nucleotides mRNA_4610: 12978 - HMMPfam-Fungal Zn(2)-Cys(6) binuclear cluster domain-PF00172,HMMPfam-Fungal specific transcription factor domain-PF04082,ProSiteProfiles-Zn(2)-C6 fungal-type DNA-binding domain profile.-PS50048,SMART-GAL4-like Zn(II)2Cys6 (or C6 zinc) binuclear cluster DNA-binding domain-SM00066,SMART-Fungal specific transcription factor domain-SM00906,SUPERFAMILY--SSF57701, at 578,505 to 580,784 12978 mRNA_4611: 12979 - K07652 vicK two-component system, OmpR family, sensor histidine kinase VicK, at 581,110 to 587,880 12979 mRNA_4612: 12980 - KOG0001 Ubiquitin and ubiquitin-like proteins, at 594,081 to 594,633 12980 mRNA_4613: 12981 - BLAST phospholipid-translocating ATPase [Microbotryum lychn..., at 598,191 to 598,847 12981 mRNA_4614: 12982 - BLAST B-cell lymphoma/leukemia 11B [Orchesella cincta], at 599,897 to 601,386 12982
Group Condition mRNA_4610 mRNA_4611 mRNA_4612 mRNA_4613 mRNA_4614
12978 12979 12980 12981 12982
ypd buoyancy 40 hrs No Buoyancy Separation -0.7 -0.1 N.D. -0.7 -0.8
buoyancy on lipid accumulation 40 hrs Low Buoyancy +0.0 -0.3 N.D. -0.3 -0.5
buoyancy on lipid accumulation 40 hrs Median Buoyancy -0.1 -0.1 N.D. -0.3 -0.4
ypd buoyancy 40 hrs Low Buoyancy +0.2 -0.3 N.D. +0.0 -0.7
ypd buoyancy 40 hrs High Buoyancy +0.3 -0.0 N.D. -0.2 -0.7
facs on lipid accumulation Sorted High BODIPY Gate -0.3 -0.3 N.D. +0.2 -0.3
auxotrophy YNB Glucose 75mM Methionine +0.1 -0.0 N.D. +0.1 -0.8
buoyancy on lipid accumulation 40 hrs High Buoyancy -0.3 -0.1 N.D. -0.1 +0.0
ypd buoyancy 40 hrs Median Buoyancy +0.0 -0.1 N.D. +0.2 -0.3
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation +0.0 -0.2 N.D. +0.3 -0.1
auxotrophy Dropout Complete +0.0 -0.1 N.D. -0.0 +0.1
auxotrophy YNB Glucose +0.0 -0.1 N.D. +0.2 -0.1
facs on lipid accumulation Sorted No BODIPY Gate -0.2 +0.1 N.D. +0.4 -0.1
growth on oleic acid YNB Oleic Acid +0.1 -0.1 N.D. +0.1 -0.0
auxotrophy YNB Glucose 75mM Arginine +0.2 +0.0 N.D. -0.1 +0.1
growth on ypd YPD +0.2 -0.1 N.D. -0.1 +0.3
growth on ricinoleic acid YNB Methylricinoleic Acid +0.4 -0.2 N.D. +0.1 +0.0
growth on ricinoleic acid YNB Glucose +0.3 -0.2 N.D. +0.1 +0.1
facs on lipid accumulation Sorted Low BODIPY Gate -0.3 +0.4 N.D. +0.3 +0.2
growth on oleic acid YNB Glucose +0.0 +0.0 N.D. +0.4 +0.4
growth on ricinoleic acid YNB Ricinoleic Acid +0.4 -0.2 N.D. +0.4 +0.4
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