Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 12987 and 12988 are separated by 1434 nucleotides12988 and 12989 are separated by 124 nucleotides12989 and 12990 are separated by 324 nucleotides12990 and 12991 are separated by 373 nucleotides mRNA_4619: 12987 - KOG2062 26S proteasome regulatory complex, subunit RPN2/PSMD1, at 615,646 to 616,230 12987 mRNA_4620: 12988 - K05293 PIGU phosphatidylinositol glycan, class U, at 617,665 to 619,015 12988 mRNA_4621: 12989 - KOG0141 Isovaleryl-CoA dehydrogenase, at 619,140 to 621,311 12989 mRNA_4622: 12990 - K20168 TBC1D15 TBC1 domain family member 15, at 621,636 to 624,863 12990 mRNA_4623: 12991 - HMMPfam-F-box domain-PF00646,ProSiteProfiles-F-box domain profile.-PS50181,SMART-A Receptor for Ubiquitination Targets-SM00256,SUPERFAMILY--SSF81383, at 625,237 to 628,203 12991
Group Condition mRNA_4619 mRNA_4620 mRNA_4621 mRNA_4622 mRNA_4623
12987 12988 12989 12990 12991
facs on lipid accumulation Sorted Low BODIPY Gate N.D. N.D. -0.6 -0.8 +0.3
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation N.D. N.D. -0.1 -0.7 -0.1
ypd buoyancy 40 hrs No Buoyancy Separation N.D. N.D. -1.0 +0.0 +0.3
auxotrophy Dropout Complete N.D. N.D. -0.4 +0.4 -0.6
facs on lipid accumulation Sorted High BODIPY Gate N.D. N.D. -0.3 -0.4 +0.1
facs on lipid accumulation Sorted No BODIPY Gate N.D. N.D. -0.2 -0.1 -0.2
ypd buoyancy 40 hrs Low Buoyancy N.D. N.D. -0.1 -0.0 -0.1
growth on ricinoleic acid YNB Glucose N.D. N.D. -0.1 +0.1 -0.1
buoyancy on lipid accumulation 40 hrs Median Buoyancy N.D. N.D. +0.2 -0.4 +0.1
growth on ypd YPD N.D. N.D. +0.9 -0.7 -0.2
buoyancy on lipid accumulation 40 hrs Low Buoyancy N.D. N.D. +0.2 -0.1 -0.1
auxotrophy YNB Glucose 75mM Arginine N.D. N.D. -0.4 +0.5 -0.1
growth on oleic acid YNB Oleic Acid N.D. N.D. -0.2 +0.5 -0.1
growth on ricinoleic acid YNB Methylricinoleic Acid N.D. N.D. -0.0 +0.3 -0.1
auxotrophy YNB Glucose N.D. N.D. -0.2 +0.4 +0.0
ypd buoyancy 40 hrs High Buoyancy N.D. N.D. -0.1 +0.5 -0.1
ypd buoyancy 40 hrs Median Buoyancy N.D. N.D. -0.2 +0.3 +0.2
buoyancy on lipid accumulation 40 hrs High Buoyancy N.D. N.D. +0.4 -0.2 +0.1
growth on ricinoleic acid YNB Ricinoleic Acid N.D. N.D. +0.2 +0.3 -0.1
growth on oleic acid YNB Glucose N.D. N.D. -0.2 +0.3 +0.3
auxotrophy YNB Glucose 75mM Methionine N.D. N.D. +0.3 +0.6 -0.0
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