Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 13000 and 13001 are separated by 99 nucleotides13001 and 13002 are separated by 1643 nucleotides13002 and 13003 are separated by 237 nucleotides13003 and 13004 are separated by 167 nucleotides mRNA_4632: 13000 - K13107 RBMX2, IST3 RNA-binding motif protein, X-linked 2, at 651,672 to 652,888 13000 mRNA_4633: 13001 - K00521 E1.16.1.7 ferric-chelate reductase, at 652,988 to 655,146 13001 mRNA_4634: 13002 - K00873 PK, pyk pyruvate kinase, at 656,790 to 659,276 13002 mRNA_4635: 13003 - Hypothetical Protein, at 659,514 to 660,743 13003 mRNA_4636: 13004 - BLAST fungal specific transcription protein, putative [Rhiz..., at 660,911 to 661,323 13004
Group Condition mRNA_4632 mRNA_4633 mRNA_4634 mRNA_4635 mRNA_4636
13000 13001 13002 13003 13004
ypd buoyancy 40 hrs No Buoyancy Separation -0.6 -0.4 N.D. -0.3 -0.0
facs on lipid accumulation Sorted Low BODIPY Gate -0.3 -0.4 N.D. +0.3 -0.3
buoyancy on lipid accumulation 40 hrs Low Buoyancy +0.3 -0.0 N.D. -0.4 -0.1
growth on oleic acid YNB Oleic Acid -0.5 -0.5 N.D. +0.4 +0.5
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation +0.1 -0.1 N.D. +0.0 -0.1
ypd buoyancy 40 hrs High Buoyancy -0.4 -0.1 N.D. +0.7 -0.1
buoyancy on lipid accumulation 40 hrs Median Buoyancy +0.4 -0.0 N.D. -0.1 -0.1
facs on lipid accumulation Sorted High BODIPY Gate -0.1 +0.1 N.D. -0.1 +0.3
growth on oleic acid YNB Glucose -0.5 -0.3 N.D. +0.5 +0.5
growth on ypd YPD +0.1 +0.3 N.D. +0.4 -0.5
ypd buoyancy 40 hrs Median Buoyancy +0.5 -0.2 N.D. +0.2 -0.1
auxotrophy YNB Glucose 75mM Methionine +0.6 -0.2 N.D. -0.1 +0.2
growth on ricinoleic acid YNB Methylricinoleic Acid +0.3 +0.2 N.D. -0.1 +0.1
auxotrophy Dropout Complete +0.2 +0.1 N.D. -0.1 +0.2
buoyancy on lipid accumulation 40 hrs High Buoyancy +0.8 -0.0 N.D. -0.3 +0.1
auxotrophy YNB Glucose +0.7 -0.1 N.D. -0.2 +0.2
growth on ricinoleic acid YNB Glucose +0.4 +0.6 N.D. +0.0 -0.4
ypd buoyancy 40 hrs Low Buoyancy +0.3 -0.1 N.D. +0.3 +0.3
growth on ricinoleic acid YNB Ricinoleic Acid +0.9 +0.3 N.D. +0.1 -0.1
auxotrophy YNB Glucose 75mM Arginine +1.0 +0.1 N.D. +0.0 +0.3
facs on lipid accumulation Sorted No BODIPY Gate +0.9 +0.4 N.D. +0.2 -0.1
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