Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 13005 and 13006 are separated by 93 nucleotides13006 and 13007 are separated by 130 nucleotides13007 and 13008 are separated by 266 nucleotides13008 and 13009 are separated by 393 nucleotides mRNA_4637: 13005 - Hypothetical Protein, at 661,764 to 663,098 13005 mRNA_4638: 13006 - HMMPfam-GDSL/SGNH-like Acyl-Esterase family found in Pmr5 and Cas1p-PF13839,SUPERFAMILY--SSF52266, at 663,192 to 664,874 13006 mRNA_4639: 13007 - KOG3469 Cytochrome c oxidase, subunit VIa/COX13, at 665,005 to 666,466 13007 mRNA_4640: 13008 - KOG1399 Flavin-containing monooxygenase, at 666,733 to 668,799 13008 mRNA_4641: 13009 - KOG1161 Protein involved in vacuolar polyphosphate accumulation, contains SPX domain, at 669,193 to 672,697 13009
Group Condition mRNA_4637 mRNA_4638 mRNA_4639 mRNA_4640 mRNA_4641
13005 13006 13007 13008 13009
ypd buoyancy 40 hrs No Buoyancy Separation -0.6 +0.3 -0.1 -0.7 -2.3
growth on ricinoleic acid YNB Glucose +0.0 -0.4 -0.2 -0.6 -1.5
growth on ricinoleic acid YNB Methylricinoleic Acid +0.2 -0.4 -0.5 -0.2 -1.3
growth on ricinoleic acid YNB Ricinoleic Acid -0.1 -0.1 -0.3 -0.4 -0.7
auxotrophy YNB Glucose +0.7 -0.1 +0.3 -0.4 -1.6
auxotrophy Dropout Complete +0.7 +0.1 +0.0 -0.3 -1.5
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation -0.0 -0.2 +0.1 -0.2 -0.5
facs on lipid accumulation Sorted Low BODIPY Gate +0.4 +0.1 +0.6 -0.6 -1.4
growth on oleic acid YNB Oleic Acid +0.2 +0.3 -0.1 -0.2 -1.1
growth on oleic acid YNB Glucose +0.1 +0.2 +0.4 +0.0 -1.5
ypd buoyancy 40 hrs Low Buoyancy -0.1 +0.2 -0.0 -0.1 -0.7
buoyancy on lipid accumulation 40 hrs Low Buoyancy +0.5 +0.0 -0.1 -0.2 -1.0
ypd buoyancy 40 hrs Median Buoyancy -0.2 +0.3 +0.1 +0.1 -0.7
ypd buoyancy 40 hrs High Buoyancy +0.3 +0.2 +0.0 +0.0 -0.9
auxotrophy YNB Glucose 75mM Methionine +0.8 +0.0 +0.3 -0.1 -1.3
auxotrophy YNB Glucose 75mM Arginine +0.8 +0.0 +0.1 -0.2 -1.0
buoyancy on lipid accumulation 40 hrs Median Buoyancy +0.2 +0.1 +0.2 -0.1 -0.5
facs on lipid accumulation Sorted No BODIPY Gate +0.3 +0.3 +0.5 -0.1 -0.5
growth on ypd YPD -0.4 -0.1 +0.4 -0.1 +0.7
facs on lipid accumulation Sorted High BODIPY Gate +0.4 -0.2 +0.4 +0.0 -0.0
buoyancy on lipid accumulation 40 hrs High Buoyancy +0.0 +0.3 +0.1 -0.2 +0.4
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