Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 13018 and 13019 are separated by 439 nucleotides13019 and 13020 are separated by 58 nucleotides13020 and 13021 are separated by 1137 nucleotides13021 and 13022 are separated by 149 nucleotides mRNA_4650: 13018 - K02919 RP-L36, MRPL36, rpmJ large subunit ribosomal protein L36, at 695,007 to 695,565 13018 mRNA_4651: 13019 - Hypothetical Protein, at 696,005 to 696,627 13019 mRNA_4652: 13020 - Hypothetical Protein, at 696,686 to 697,396 13020 mRNA_4653: 13021 - KOG1203 Predicted dehydrogenase, at 698,534 to 699,580 13021 mRNA_4654: 13022 - KOG4253 Tryptophan-rich basic nuclear protein, at 699,730 to 700,546 13022
Group Condition mRNA_4650 mRNA_4651 mRNA_4652 mRNA_4653 mRNA_4654
13018 13019 13020 13021 13022
facs on lipid accumulation Sorted High BODIPY Gate -0.3 N.D. -0.1 +0.0 -1.7
ypd buoyancy 40 hrs No Buoyancy Separation +0.2 N.D. -0.2 -0.1 -1.0
facs on lipid accumulation Sorted Low BODIPY Gate -0.7 N.D. -0.0 +0.2 -0.4
auxotrophy YNB Glucose 75mM Methionine +0.4 N.D. -0.2 -0.0 -0.8
growth on ricinoleic acid YNB Methylricinoleic Acid -0.3 N.D. +0.4 +0.3 -1.0
auxotrophy YNB Glucose +0.2 N.D. -0.0 +0.0 -0.5
facs on lipid accumulation Sorted No BODIPY Gate -0.1 N.D. -0.6 +0.2 +0.2
growth on ricinoleic acid YNB Glucose -0.2 N.D. +0.1 +0.1 -0.2
ypd buoyancy 40 hrs Low Buoyancy -0.0 N.D. +0.3 -0.1 -0.2
buoyancy on lipid accumulation 40 hrs High Buoyancy -0.1 N.D. -0.4 +0.1 +0.2
growth on ricinoleic acid YNB Ricinoleic Acid -0.0 N.D. +0.4 +0.3 -0.6
auxotrophy YNB Glucose 75mM Arginine +0.7 N.D. -0.2 +0.2 -0.6
growth on oleic acid YNB Oleic Acid +0.3 N.D. +0.1 +0.3 -0.4
ypd buoyancy 40 hrs High Buoyancy -0.0 N.D. +0.6 +0.1 -0.4
growth on oleic acid YNB Glucose +0.5 N.D. -0.2 +0.2 -0.0
growth on ypd YPD +0.1 N.D. +0.9 -0.2 -0.3
ypd buoyancy 40 hrs Median Buoyancy -0.1 N.D. +0.2 -0.1 +0.6
auxotrophy Dropout Complete +0.5 N.D. +0.3 +0.2 -0.0
buoyancy on lipid accumulation 40 hrs Low Buoyancy +0.6 N.D. -0.4 +0.3 +1.2
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation +0.5 N.D. -0.1 +0.1 +1.4
buoyancy on lipid accumulation 40 hrs Median Buoyancy +0.7 N.D. -0.2 +0.2 +1.7
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