Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 13020 and 13021 are separated by 1137 nucleotides13021 and 13022 are separated by 149 nucleotides13022 and 13023 are separated by 221 nucleotides13023 and 13024 are separated by 257 nucleotides mRNA_4652: 13020 - Hypothetical Protein, at 696,686 to 697,396 13020 mRNA_4653: 13021 - KOG1203 Predicted dehydrogenase, at 698,534 to 699,580 13021 mRNA_4654: 13022 - KOG4253 Tryptophan-rich basic nuclear protein, at 699,730 to 700,546 13022 mRNA_4655: 13023 - K03124 TFIIB, GTF2B, SUA7, tfb transcription initiation factor TFIIB, at 700,768 to 702,328 13023 mRNA_4656: 13024 - Hypothetical Protein, at 702,586 to 704,357 13024
Group Condition mRNA_4652 mRNA_4653 mRNA_4654 mRNA_4655 mRNA_4656
13020 13021 13022 13023 13024
facs on lipid accumulation Sorted High BODIPY Gate -0.1 +0.0 -1.7 N.D. +0.1
ypd buoyancy 40 hrs No Buoyancy Separation -0.2 -0.1 -1.0 N.D. -0.0
auxotrophy YNB Glucose 75mM Methionine -0.2 -0.0 -0.8 N.D. +0.1
facs on lipid accumulation Sorted Low BODIPY Gate -0.0 +0.2 -0.4 N.D. -0.1
auxotrophy YNB Glucose -0.0 +0.0 -0.5 N.D. +0.2
auxotrophy YNB Glucose 75mM Arginine -0.2 +0.2 -0.6 N.D. +0.3
ypd buoyancy 40 hrs Low Buoyancy +0.3 -0.1 -0.2 N.D. +0.1
growth on oleic acid YNB Glucose -0.2 +0.2 -0.0 N.D. +0.0
growth on oleic acid YNB Oleic Acid +0.1 +0.3 -0.4 N.D. +0.1
growth on ricinoleic acid YNB Methylricinoleic Acid +0.4 +0.3 -1.0 N.D. +0.3
facs on lipid accumulation Sorted No BODIPY Gate -0.6 +0.2 +0.2 N.D. +0.4
buoyancy on lipid accumulation 40 hrs High Buoyancy -0.4 +0.1 +0.2 N.D. +0.3
growth on ypd YPD +0.9 -0.2 -0.3 N.D. -0.0
ypd buoyancy 40 hrs High Buoyancy +0.6 +0.1 -0.4 N.D. +0.1
growth on ricinoleic acid YNB Glucose +0.1 +0.1 -0.2 N.D. +0.4
growth on ricinoleic acid YNB Ricinoleic Acid +0.4 +0.3 -0.6 N.D. +0.5
auxotrophy Dropout Complete +0.3 +0.2 -0.0 N.D. +0.2
ypd buoyancy 40 hrs Median Buoyancy +0.2 -0.1 +0.6 N.D. -0.0
buoyancy on lipid accumulation 40 hrs Low Buoyancy -0.4 +0.3 +1.2 N.D. +0.1
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation -0.1 +0.1 +1.4 N.D. +0.0
buoyancy on lipid accumulation 40 hrs Median Buoyancy -0.2 +0.2 +1.7 N.D. +0.0
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