Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 13037 and 13038 are separated by 251 nucleotides13038 and 13039 are separated by 200 nucleotides13039 and 13040 are separated by 472 nucleotides13040 and 13041 are separated by 379 nucleotides mRNA_4669: 13037 - K12627 LSM8 U6 snRNA-associated Sm-like protein LSm8, at 738,186 to 738,564 13037 mRNA_4670: 13038 - Hypothetical Protein, at 738,816 to 739,415 13038 mRNA_4671: 13039 - Hypothetical Protein, at 739,616 to 741,558 13039 mRNA_4672: 13040 - K11979 UBR7 E3 ubiquitin-protein ligase UBR7, at 742,031 to 743,506 13040 mRNA_4673: 13041 - K01069 E3.1.2.6, gloB hydroxyacylglutathione hydrolase, at 743,886 to 745,346 13041
Group Condition mRNA_4669 mRNA_4670 mRNA_4671 mRNA_4672 mRNA_4673
13037 13038 13039 13040 13041
facs on lipid accumulation Sorted Low BODIPY Gate N.D. -0.1 -1.1 +0.5 -1.9
growth on ricinoleic acid YNB Ricinoleic Acid N.D. -0.4 -0.1 +0.2 +0.2
growth on ypd YPD N.D. +0.7 -0.2 -0.1 -0.1
ypd buoyancy 40 hrs High Buoyancy N.D. +0.7 +0.1 +0.9 -1.3
ypd buoyancy 40 hrs No Buoyancy Separation N.D. +1.2 -0.1 +1.4 -2.0
facs on lipid accumulation Sorted No BODIPY Gate N.D. +0.4 -0.4 +0.1 +0.6
growth on ricinoleic acid YNB Methylricinoleic Acid N.D. +0.4 -0.1 +0.2 +0.2
facs on lipid accumulation Sorted High BODIPY Gate N.D. -0.5 +0.5 +0.8 +0.1
growth on oleic acid YNB Oleic Acid N.D. +0.3 -0.4 -0.1 +1.1
growth on ricinoleic acid YNB Glucose N.D. +0.5 +0.1 +0.4 +0.1
buoyancy on lipid accumulation 40 hrs Low Buoyancy N.D. +0.2 +0.8 +0.2 +0.1
buoyancy on lipid accumulation 40 hrs Median Buoyancy N.D. -0.2 +0.9 +0.1 +0.5
ypd buoyancy 40 hrs Median Buoyancy N.D. +0.8 +0.3 +0.9 -0.3
growth on oleic acid YNB Glucose N.D. +0.5 -0.0 +0.5 +1.0
ypd buoyancy 40 hrs Low Buoyancy N.D. +1.4 +0.4 +0.4 -0.2
auxotrophy YNB Glucose 75mM Methionine N.D. +2.2 +0.2 +0.2 -0.4
buoyancy on lipid accumulation 40 hrs High Buoyancy N.D. +0.2 +1.1 +0.4 +0.8
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation N.D. +0.0 +1.3 -0.0 +1.4
auxotrophy YNB Glucose N.D. +2.0 +0.6 +0.0 +0.5
auxotrophy Dropout Complete N.D. +2.8 -0.1 +0.3 +0.3
auxotrophy YNB Glucose 75mM Arginine N.D. +2.0 +0.4 +0.4 +0.9
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