Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 13040 and 13041 are separated by 379 nucleotides13041 and 13042 are separated by 525 nucleotides13042 and 13043 are separated by 191 nucleotides13043 and 13044 are separated by 360 nucleotides mRNA_4672: 13040 - K11979 UBR7 E3 ubiquitin-protein ligase UBR7, at 742,031 to 743,506 13040 mRNA_4673: 13041 - K01069 E3.1.2.6, gloB hydroxyacylglutathione hydrolase, at 743,886 to 745,346 13041 mRNA_4674: 13042 - K08150 SLC2A13, ITR MFS transporter, SP family, solute carrier family 2 (myo-inositol transporter), member 13, at 745,872 to 747,839 13042 mRNA_4675: 13043 - SUPERFAMILY--SSF53448, at 748,031 to 749,259 13043 mRNA_4676: 13044 - K01101 E3.1.3.41 4-nitrophenyl phosphatase, at 749,620 to 750,873 13044
Group Condition mRNA_4672 mRNA_4673 mRNA_4674 mRNA_4675 mRNA_4676
13040 13041 13042 13043 13044
facs on lipid accumulation Sorted Low BODIPY Gate +0.5 -1.9 +0.3 N.D. N.D.
auxotrophy YNB Glucose 75mM Methionine +0.2 -0.4 -0.7 N.D. N.D.
ypd buoyancy 40 hrs No Buoyancy Separation +1.4 -2.0 -0.1 N.D. N.D.
growth on ypd YPD -0.1 -0.1 +0.1 N.D. N.D.
ypd buoyancy 40 hrs High Buoyancy +0.9 -1.3 +0.5 N.D. N.D.
growth on ricinoleic acid YNB Ricinoleic Acid +0.2 +0.2 -0.2 N.D. N.D.
auxotrophy YNB Glucose +0.0 +0.5 -0.3 N.D. N.D.
auxotrophy Dropout Complete +0.3 +0.3 -0.4 N.D. N.D.
growth on ricinoleic acid YNB Methylricinoleic Acid +0.2 +0.2 -0.2 N.D. N.D.
buoyancy on lipid accumulation 40 hrs Low Buoyancy +0.2 +0.1 +0.1 N.D. N.D.
ypd buoyancy 40 hrs Low Buoyancy +0.4 -0.2 +0.3 N.D. N.D.
buoyancy on lipid accumulation 40 hrs Median Buoyancy +0.1 +0.5 -0.1 N.D. N.D.
growth on ricinoleic acid YNB Glucose +0.4 +0.1 +0.1 N.D. N.D.
growth on oleic acid YNB Oleic Acid -0.1 +1.1 -0.3 N.D. N.D.
facs on lipid accumulation Sorted No BODIPY Gate +0.1 +0.6 +0.2 N.D. N.D.
ypd buoyancy 40 hrs Median Buoyancy +0.9 -0.3 +0.3 N.D. N.D.
facs on lipid accumulation Sorted High BODIPY Gate +0.8 +0.1 +0.0 N.D. N.D.
auxotrophy YNB Glucose 75mM Arginine +0.4 +0.9 -0.3 N.D. N.D.
growth on oleic acid YNB Glucose +0.5 +1.0 -0.5 N.D. N.D.
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation -0.0 +1.4 -0.1 N.D. N.D.
buoyancy on lipid accumulation 40 hrs High Buoyancy +0.4 +0.8 +0.2 N.D. N.D.
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