Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 13044 and 13045 are separated by 441 nucleotides13045 and 13046 are separated by 308 nucleotides13046 and 13047 are separated by 962 nucleotides13047 and 13048 are separated by 212 nucleotides mRNA_4676: 13044 - K01101 E3.1.3.41 4-nitrophenyl phosphatase, at 749,620 to 750,873 13044 mRNA_4677: 13045 - K10426 DCTN4 dynactin 4, at 751,315 to 753,347 13045 mRNA_4678: 13046 - K11252 H2B histone H2B, at 753,656 to 754,294 13046 mRNA_4679: 13047 - Hypothetical Protein, at 755,257 to 756,395 13047 mRNA_4680: 13048 - K01067 E3.1.2.1, ACH1 acetyl-CoA hydrolase, at 756,608 to 758,345 13048
Group Condition mRNA_4676 mRNA_4677 mRNA_4678 mRNA_4679 mRNA_4680
13044 13045 13046 13047 13048
growth on oleic acid YNB Glucose N.D. N.D. -0.3 -0.4 -0.2
auxotrophy YNB Glucose 75mM Methionine N.D. N.D. -0.2 -0.2 -0.2
growth on oleic acid YNB Oleic Acid N.D. N.D. -0.0 -0.3 -0.2
auxotrophy YNB Glucose N.D. N.D. -0.2 -0.3 +0.0
ypd buoyancy 40 hrs Median Buoyancy N.D. N.D. -0.0 -0.2 -0.0
ypd buoyancy 40 hrs High Buoyancy N.D. N.D. +0.1 -0.4 +0.0
ypd buoyancy 40 hrs Low Buoyancy N.D. N.D. -0.1 -0.1 +0.1
facs on lipid accumulation Sorted High BODIPY Gate N.D. N.D. -0.1 -0.3 +0.4
auxotrophy YNB Glucose 75mM Arginine N.D. N.D. +0.2 -0.0 -0.1
facs on lipid accumulation Sorted No BODIPY Gate N.D. N.D. +0.2 -0.1 -0.1
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation N.D. N.D. +0.6 -0.2 -0.3
auxotrophy Dropout Complete N.D. N.D. +0.2 -0.2 +0.1
ypd buoyancy 40 hrs No Buoyancy Separation N.D. N.D. +0.1 -0.0 +0.0
growth on ricinoleic acid YNB Ricinoleic Acid N.D. N.D. +0.5 +0.0 -0.2
facs on lipid accumulation Sorted Low BODIPY Gate N.D. N.D. +0.4 -0.2 +0.1
growth on ricinoleic acid YNB Methylricinoleic Acid N.D. N.D. +0.3 +0.0 +0.2
growth on ricinoleic acid YNB Glucose N.D. N.D. +0.4 +0.1 +0.1
buoyancy on lipid accumulation 40 hrs Median Buoyancy N.D. N.D. +0.5 +0.2 +0.3
buoyancy on lipid accumulation 40 hrs High Buoyancy N.D. N.D. +0.5 +0.1 +0.3
buoyancy on lipid accumulation 40 hrs Low Buoyancy N.D. N.D. +0.7 -0.1 +0.3
growth on ypd YPD N.D. N.D. -0.3 +0.7 +0.5
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