Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 13045 and 13046 are separated by 308 nucleotides13046 and 13047 are separated by 962 nucleotides13047 and 13048 are separated by 212 nucleotides13048 and 13049 are separated by 1254 nucleotides mRNA_4677: 13045 - K10426 DCTN4 dynactin 4, at 751,315 to 753,347 13045 mRNA_4678: 13046 - K11252 H2B histone H2B, at 753,656 to 754,294 13046 mRNA_4679: 13047 - Hypothetical Protein, at 755,257 to 756,395 13047 mRNA_4680: 13048 - K01067 E3.1.2.1, ACH1 acetyl-CoA hydrolase, at 756,608 to 758,345 13048 mRNA_4681: 13049 - K01539 ATP1A sodium/potassium-transporting ATPase subunit alpha, at 759,600 to 763,345 13049
Group Condition mRNA_4677 mRNA_4678 mRNA_4679 mRNA_4680 mRNA_4681
13045 13046 13047 13048 13049
growth on oleic acid YNB Glucose N.D. -0.3 -0.4 -0.2 -0.1
auxotrophy YNB Glucose 75mM Methionine N.D. -0.2 -0.2 -0.2 -0.4
auxotrophy YNB Glucose N.D. -0.2 -0.3 +0.0 -0.3
growth on oleic acid YNB Oleic Acid N.D. -0.0 -0.3 -0.2 -0.0
auxotrophy YNB Glucose 75mM Arginine N.D. +0.2 -0.0 -0.1 -0.4
facs on lipid accumulation Sorted High BODIPY Gate N.D. -0.1 -0.3 +0.4 -0.3
ypd buoyancy 40 hrs Median Buoyancy N.D. -0.0 -0.2 -0.0 -0.0
ypd buoyancy 40 hrs High Buoyancy N.D. +0.1 -0.4 +0.0 +0.0
ypd buoyancy 40 hrs Low Buoyancy N.D. -0.1 -0.1 +0.1 -0.0
facs on lipid accumulation Sorted No BODIPY Gate N.D. +0.2 -0.1 -0.1 -0.2
ypd buoyancy 40 hrs No Buoyancy Separation N.D. +0.1 -0.0 +0.0 -0.2
auxotrophy Dropout Complete N.D. +0.2 -0.2 +0.1 -0.1
growth on ricinoleic acid YNB Methylricinoleic Acid N.D. +0.3 +0.0 +0.2 -0.3
facs on lipid accumulation Sorted Low BODIPY Gate N.D. +0.4 -0.2 +0.1 +0.0
growth on ricinoleic acid YNB Ricinoleic Acid N.D. +0.5 +0.0 -0.2 +0.2
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation N.D. +0.6 -0.2 -0.3 +0.4
growth on ricinoleic acid YNB Glucose N.D. +0.4 +0.1 +0.1 +0.1
growth on ypd YPD N.D. -0.3 +0.7 +0.5 -0.1
buoyancy on lipid accumulation 40 hrs High Buoyancy N.D. +0.5 +0.1 +0.3 +0.2
buoyancy on lipid accumulation 40 hrs Median Buoyancy N.D. +0.5 +0.2 +0.3 +0.5
buoyancy on lipid accumulation 40 hrs Low Buoyancy N.D. +0.7 -0.1 +0.3 +0.5
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