Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 13059 and 13060 are separated by 276 nucleotides13060 and 13061 are separated by 646 nucleotides13061 and 13062 are separated by 2021 nucleotides13062 and 13063 are separated by 328 nucleotides mRNA_4691: 13059 - Hypothetical Protein, at 787,368 to 788,152 13059 mRNA_4692: 13060 - K00559 E2.1.1.41, SMT1, ERG6 sterol 24-C-methyltransferase, at 788,429 to 789,828 13060 mRNA_4693: 13061 - Hypothetical Protein, at 790,475 to 790,930 13061 mRNA_4694: 13062 - K11252 H2B histone H2B, at 792,952 to 793,587 13062 mRNA_4695: 13063 - K11251 H2A histone H2A, at 793,916 to 794,516 13063
Group Condition mRNA_4691 mRNA_4692 mRNA_4693 mRNA_4694 mRNA_4695
13059 13060 13061 13062 13063
facs on lipid accumulation Sorted No BODIPY Gate -0.5 N.D. -1.4 N.D. -0.3
facs on lipid accumulation Sorted High BODIPY Gate -0.8 N.D. -0.4 N.D. +0.0
ypd buoyancy 40 hrs No Buoyancy Separation +0.0 N.D. -1.0 N.D. +0.2
facs on lipid accumulation Sorted Low BODIPY Gate -0.3 N.D. -0.1 N.D. -0.1
auxotrophy YNB Glucose 75mM Arginine +0.1 N.D. -0.3 N.D. -0.3
growth on ypd YPD +0.0 N.D. -0.6 N.D. +0.3
ypd buoyancy 40 hrs Low Buoyancy -0.0 N.D. -0.5 N.D. +0.4
growth on ricinoleic acid YNB Ricinoleic Acid -0.4 N.D. +0.1 N.D. +0.2
ypd buoyancy 40 hrs High Buoyancy -0.2 N.D. -0.0 N.D. +0.2
growth on ricinoleic acid YNB Methylricinoleic Acid -0.5 N.D. +0.3 N.D. +0.3
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation +0.5 N.D. -0.1 N.D. -0.2
auxotrophy Dropout Complete +0.3 N.D. +0.0 N.D. -0.0
auxotrophy YNB Glucose 75mM Methionine +0.3 N.D. +0.0 N.D. -0.1
growth on ricinoleic acid YNB Glucose -0.5 N.D. +0.8 N.D. +0.0
growth on oleic acid YNB Glucose -0.1 N.D. +0.3 N.D. +0.2
ypd buoyancy 40 hrs Median Buoyancy +0.0 N.D. +0.3 N.D. +0.1
auxotrophy YNB Glucose +0.3 N.D. +0.8 N.D. -0.3
growth on oleic acid YNB Oleic Acid +0.2 N.D. +0.6 N.D. +0.4
buoyancy on lipid accumulation 40 hrs Low Buoyancy +0.5 N.D. +2.0 N.D. -0.3
buoyancy on lipid accumulation 40 hrs High Buoyancy +0.7 N.D. +2.0 N.D. +0.2
buoyancy on lipid accumulation 40 hrs Median Buoyancy +0.5 N.D. +2.9 N.D. -0.1
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