Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 13064 and 13065 are separated by 323 nucleotides13065 and 13066 are separated by 245 nucleotides13066 and 13067 are separated by 101 nucleotides13067 and 13068 are separated by 285 nucleotides mRNA_4696: 13064 - KOG1756 Histone 2A, at 796,428 to 796,805 13064 mRNA_4697: 13065 - K05291 PIGS phosphatidylinositol glycan, class S, at 797,129 to 800,158 13065 mRNA_4698: 13066 - K12193 VPS24, CHMP3 charged multivesicular body protein 3, at 800,404 to 801,169 13066 mRNA_4699: 13067 - K09140 TSR3 pre-rRNA-processing protein TSR3, at 801,271 to 802,584 13067 mRNA_4700: 13068 - BLAST putative mannoprotein [Rhizoctonia solani 123E], at 802,870 to 804,709 13068
Group Condition mRNA_4696 mRNA_4697 mRNA_4698 mRNA_4699 mRNA_4700
13064 13065 13066 13067 13068
ypd buoyancy 40 hrs No Buoyancy Separation N.D. -0.1 N.D. -0.7 -0.5
growth on oleic acid YNB Glucose N.D. +0.1 N.D. -0.5 -0.8
growth on ricinoleic acid YNB Glucose N.D. -0.0 N.D. -0.9 -0.4
growth on ricinoleic acid YNB Methylricinoleic Acid N.D. +0.1 N.D. -0.2 -1.0
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation N.D. +0.1 N.D. -1.1 +0.1
facs on lipid accumulation Sorted No BODIPY Gate N.D. -0.0 N.D. -0.6 -0.1
growth on oleic acid YNB Oleic Acid N.D. +0.1 N.D. -0.2 -0.2
auxotrophy YNB Glucose N.D. +0.2 N.D. -0.0 -0.3
ypd buoyancy 40 hrs Low Buoyancy N.D. -0.1 N.D. -0.3 +0.2
ypd buoyancy 40 hrs High Buoyancy N.D. -0.1 N.D. -0.4 +0.3
growth on ricinoleic acid YNB Ricinoleic Acid N.D. +0.2 N.D. +0.2 -0.6
auxotrophy YNB Glucose 75mM Methionine N.D. +0.3 N.D. +0.0 -0.4
growth on ypd YPD N.D. -0.1 N.D. +0.6 -0.5
auxotrophy Dropout Complete N.D. +0.1 N.D. +0.1 -0.2
ypd buoyancy 40 hrs Median Buoyancy N.D. -0.2 N.D. -0.1 +0.3
facs on lipid accumulation Sorted Low BODIPY Gate N.D. +0.0 N.D. +0.4 -0.2
buoyancy on lipid accumulation 40 hrs Low Buoyancy N.D. +0.2 N.D. -0.1 +0.2
auxotrophy YNB Glucose 75mM Arginine N.D. +0.0 N.D. +0.3 +0.0
buoyancy on lipid accumulation 40 hrs High Buoyancy N.D. +0.3 N.D. -0.0 +0.2
buoyancy on lipid accumulation 40 hrs Median Buoyancy N.D. +0.2 N.D. +0.1 +0.3
facs on lipid accumulation Sorted High BODIPY Gate N.D. +0.1 N.D. +0.5 +0.2
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