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Fitness for 5 genes in
Rhodosporidium toruloides IFO0880
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Top 21 experiments (either direction), sorted by average fitness
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500 nt
13065 and 13066 are separated by 245 nucleotides
13066 and 13067 are separated by 101 nucleotides
13067 and 13068 are separated by 285 nucleotides
13068 and 13069 are separated by 479 nucleotides
mRNA_4697: 13065 - K05291 PIGS phosphatidylinositol glycan, class S, at 797,129 to 800,158
13065
mRNA_4698: 13066 - K12193 VPS24, CHMP3 charged multivesicular body protein 3, at 800,404 to 801,169
13066
mRNA_4699: 13067 - K09140 TSR3 pre-rRNA-processing protein TSR3, at 801,271 to 802,584
13067
mRNA_4700: 13068 - BLAST putative mannoprotein [Rhizoctonia solani 123E], at 802,870 to 804,709
13068
mRNA_4701: 13069 - HMMPfam-Asp/Glu/Hydantoin racemase-PF01177, at 805,189 to 806,290
13069
Group
Condition
mRNA
_4697
mRNA
_4698
mRNA
_4699
mRNA
_4700
mRNA
_4701
13065
13066
13067
13068
13069
growth on ricinoleic acid
YNB Methylricinoleic Acid
+0.1
N.D.
-0.2
-1.0
-0.7
ypd buoyancy
40 hrs No Buoyancy Separation
-0.1
N.D.
-0.7
-0.5
+0.0
growth on ricinoleic acid
YNB Glucose
-0.0
N.D.
-0.9
-0.4
+0.1
growth on oleic acid
YNB Glucose
+0.1
N.D.
-0.5
-0.8
+0.4
buoyancy on lipid accumulation
40 hrs No Buoyancy Separation
+0.1
N.D.
-1.1
+0.1
+0.1
facs on lipid accumulation
Sorted No BODIPY Gate
-0.0
N.D.
-0.6
-0.1
+0.1
growth on ricinoleic acid
YNB Ricinoleic Acid
+0.2
N.D.
+0.2
-0.6
-0.5
growth on ypd
YPD
-0.1
N.D.
+0.6
-0.5
-0.4
ypd buoyancy
40 hrs High Buoyancy
-0.1
N.D.
-0.4
+0.3
-0.2
auxotrophy
YNB Glucose
+0.2
N.D.
-0.0
-0.3
+0.0
growth on oleic acid
YNB Oleic Acid
+0.1
N.D.
-0.2
-0.2
+0.1
ypd buoyancy
40 hrs Low Buoyancy
-0.1
N.D.
-0.3
+0.2
+0.2
auxotrophy
YNB Glucose 75mM Methionine
+0.3
N.D.
+0.0
-0.4
+0.2
facs on lipid accumulation
Sorted Low BODIPY Gate
+0.0
N.D.
+0.4
-0.2
-0.1
auxotrophy
Dropout Complete
+0.1
N.D.
+0.1
-0.2
+0.2
ypd buoyancy
40 hrs Median Buoyancy
-0.2
N.D.
-0.1
+0.3
+0.1
buoyancy on lipid accumulation
40 hrs Median Buoyancy
+0.2
N.D.
+0.1
+0.3
-0.2
buoyancy on lipid accumulation
40 hrs High Buoyancy
+0.3
N.D.
-0.0
+0.2
-0.1
auxotrophy
YNB Glucose 75mM Arginine
+0.0
N.D.
+0.3
+0.0
+0.1
buoyancy on lipid accumulation
40 hrs Low Buoyancy
+0.2
N.D.
-0.1
+0.2
+0.7
facs on lipid accumulation
Sorted High BODIPY Gate
+0.1
N.D.
+0.5
+0.2
+0.6
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