Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 13066 and 13067 are separated by 101 nucleotides13067 and 13068 are separated by 285 nucleotides13068 and 13069 are separated by 479 nucleotides13069 and 13070 are separated by 197 nucleotides mRNA_4698: 13066 - K12193 VPS24, CHMP3 charged multivesicular body protein 3, at 800,404 to 801,169 13066 mRNA_4699: 13067 - K09140 TSR3 pre-rRNA-processing protein TSR3, at 801,271 to 802,584 13067 mRNA_4700: 13068 - BLAST putative mannoprotein [Rhizoctonia solani 123E], at 802,870 to 804,709 13068 mRNA_4701: 13069 - HMMPfam-Asp/Glu/Hydantoin racemase-PF01177, at 805,189 to 806,290 13069 mRNA_4702: 13070 - K00870 E2.7.1.37 protein kinase, at 806,488 to 808,596 13070
Group Condition mRNA_4698 mRNA_4699 mRNA_4700 mRNA_4701 mRNA_4702
13066 13067 13068 13069 13070
growth on ricinoleic acid YNB Methylricinoleic Acid N.D. -0.2 -1.0 -0.7 +0.2
ypd buoyancy 40 hrs No Buoyancy Separation N.D. -0.7 -0.5 +0.0 -0.3
growth on ricinoleic acid YNB Glucose N.D. -0.9 -0.4 +0.1 -0.0
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation N.D. -1.1 +0.1 +0.1 -0.0
facs on lipid accumulation Sorted No BODIPY Gate N.D. -0.6 -0.1 +0.1 -0.0
growth on oleic acid YNB Glucose N.D. -0.5 -0.8 +0.4 +0.3
growth on ricinoleic acid YNB Ricinoleic Acid N.D. +0.2 -0.6 -0.5 +0.4
buoyancy on lipid accumulation 40 hrs High Buoyancy N.D. -0.0 +0.2 -0.1 -0.5
growth on ypd YPD N.D. +0.6 -0.5 -0.4 +0.1
ypd buoyancy 40 hrs High Buoyancy N.D. -0.4 +0.3 -0.2 +0.1
auxotrophy YNB Glucose N.D. -0.0 -0.3 +0.0 +0.3
growth on oleic acid YNB Oleic Acid N.D. -0.2 -0.2 +0.1 +0.4
auxotrophy YNB Glucose 75mM Methionine N.D. +0.0 -0.4 +0.2 +0.3
buoyancy on lipid accumulation 40 hrs Median Buoyancy N.D. +0.1 +0.3 -0.2 -0.0
ypd buoyancy 40 hrs Median Buoyancy N.D. -0.1 +0.3 +0.1 -0.1
facs on lipid accumulation Sorted Low BODIPY Gate N.D. +0.4 -0.2 -0.1 +0.2
buoyancy on lipid accumulation 40 hrs Low Buoyancy N.D. -0.1 +0.2 +0.7 -0.4
auxotrophy Dropout Complete N.D. +0.1 -0.2 +0.2 +0.4
ypd buoyancy 40 hrs Low Buoyancy N.D. -0.3 +0.2 +0.2 +0.4
auxotrophy YNB Glucose 75mM Arginine N.D. +0.3 +0.0 +0.1 +0.4
facs on lipid accumulation Sorted High BODIPY Gate N.D. +0.5 +0.2 +0.6 +0.5
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