Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 13073 and 13074 are separated by 343 nucleotides13074 and 13075 are separated by 355 nucleotides13075 and 13076 are separated by 247 nucleotides13076 and 13077 are separated by 340 nucleotides mRNA_4705: 13073 - K06065 NCOR2, SMRT nuclear receptor co-repressor 2, at 810,527 to 816,039 13073 mRNA_4706: 13074 - KOG0101 Molecular chaperones HSP70/HSC70, HSP70 superfamily, at 816,383 to 818,450 13074 mRNA_4707: 13075 - KOG4431 Uncharacterized protein, induced by hypoxia , at 818,806 to 819,404 13075 mRNA_4708: 13076 - BLAST expressed protein [Cryptococcus neoformans var. neofo..., at 819,652 to 821,476 13076 mRNA_4709: 13077 - Hypothetical Protein, at 821,817 to 823,479 13077
Group Condition mRNA_4705 mRNA_4706 mRNA_4707 mRNA_4708 mRNA_4709
13073 13074 13075 13076 13077
facs on lipid accumulation Sorted No BODIPY Gate -0.6 N.D. -0.1 -4.7 -0.3
facs on lipid accumulation Sorted High BODIPY Gate -0.7 N.D. -0.2 -3.7 -0.3
facs on lipid accumulation Sorted Low BODIPY Gate -1.0 N.D. -0.1 -3.5 +0.3
ypd buoyancy 40 hrs No Buoyancy Separation -0.3 N.D. -3.1 -0.2 -0.3
growth on ricinoleic acid YNB Ricinoleic Acid -1.3 N.D. -0.9 -0.7 -0.9
growth on oleic acid YNB Oleic Acid -0.8 N.D. -0.2 -0.7 -0.8
growth on ricinoleic acid YNB Methylricinoleic Acid -1.3 N.D. +0.6 -0.6 -1.0
growth on ricinoleic acid YNB Glucose -1.0 N.D. -0.4 -0.1 -0.8
auxotrophy YNB Glucose 75mM Arginine -0.4 N.D. -0.5 -0.3 -0.8
auxotrophy Dropout Complete -0.4 N.D. +0.1 -0.4 -1.3
buoyancy on lipid accumulation 40 hrs Median Buoyancy +0.2 N.D. -1.3 -0.8 -0.1
growth on oleic acid YNB Glucose -0.4 N.D. +0.4 -0.2 -1.1
ypd buoyancy 40 hrs Low Buoyancy -0.4 N.D. -1.0 +0.0 +0.1
auxotrophy YNB Glucose -0.2 N.D. -0.6 +0.0 -0.4
buoyancy on lipid accumulation 40 hrs Low Buoyancy -0.4 N.D. +0.3 -0.6 -0.3
auxotrophy YNB Glucose 75mM Methionine -0.3 N.D. +0.1 -0.3 -0.6
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation +0.2 N.D. +0.4 -0.9 -0.2
ypd buoyancy 40 hrs High Buoyancy +0.0 N.D. -0.6 +0.1 +0.1
buoyancy on lipid accumulation 40 hrs High Buoyancy +0.4 N.D. +0.2 -0.9 +0.0
ypd buoyancy 40 hrs Median Buoyancy -0.1 N.D. +0.5 -0.0 +0.1
growth on ypd YPD +0.1 N.D. +1.3 -0.4 -0.4
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