Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 13078 and 13079 are separated by 328 nucleotides13079 and 13080 are separated by 232 nucleotides13080 and 13081 are separated by 334 nucleotides13081 and 13082 are separated by 124 nucleotides mRNA_4710: 13078 - BLAST putative mannoprotein [Rhizoctonia solani 123E], at 824,184 to 826,218 13078 mRNA_4711: 13079 - KOG1001 Helicase-like transcription factor HLTF/DNA helicase RAD5, DEAD-box superfamily, at 826,547 to 830,720 13079 mRNA_4712: 13080 - HMMPfam-N-terminal C2 in EEIG1 and EHBP1 proteins-PF10358, at 830,953 to 834,055 13080 mRNA_4713: 13081 - HMMPfam-Fungal Zn(2)-Cys(6) binuclear cluster domain-PF00172,HMMPfam-Fungal specific transcription factor domain-PF04082,ProSitePatterns-Zn(2)-C6 fungal-type DNA-binding domain signature.-PS00463,ProSiteProfiles-Zn(2)-C6 fungal-type DNA-binding domain profile.-PS50048,SMART-GAL4-like Zn(II)2Cys6 (or C6 zinc) binuclear cluster DNA-binding domain-SM00066,SUPERFAMILY--SSF57701, at 834,390 to 838,234 13081 mRNA_4714: 13082 - K01183 E3.2.1.14 chitinase, at 838,359 to 840,435 13082
Group Condition mRNA_4710 mRNA_4711 mRNA_4712 mRNA_4713 mRNA_4714
13078 13079 13080 13081 13082
ypd buoyancy 40 hrs No Buoyancy Separation +0.5 +0.3 -0.5 -0.5 -1.7
facs on lipid accumulation Sorted Low BODIPY Gate -0.1 +0.0 -0.2 -0.6 -0.2
facs on lipid accumulation Sorted No BODIPY Gate -0.0 -0.0 +0.0 -0.3 -0.7
buoyancy on lipid accumulation 40 hrs Low Buoyancy +0.1 -0.2 -0.1 -0.4 -0.0
growth on ricinoleic acid YNB Ricinoleic Acid +0.3 +0.3 -0.3 -0.1 -0.6
ypd buoyancy 40 hrs Low Buoyancy -0.1 +0.3 +0.1 -0.4 -0.1
growth on ypd YPD -0.6 -0.0 +0.4 -0.2 +0.3
facs on lipid accumulation Sorted High BODIPY Gate -0.1 -0.0 -0.1 -0.0 +0.3
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation +0.7 -0.2 -0.2 -0.1 -0.1
ypd buoyancy 40 hrs Median Buoyancy +0.1 +0.0 -0.0 +0.0 -0.0
growth on oleic acid YNB Oleic Acid +0.4 -0.1 -0.2 -0.1 +0.2
buoyancy on lipid accumulation 40 hrs Median Buoyancy +0.6 -0.1 +0.0 +0.0 -0.4
buoyancy on lipid accumulation 40 hrs High Buoyancy +0.4 -0.2 -0.1 +0.1 +0.1
growth on oleic acid YNB Glucose +0.3 -0.0 +0.4 -0.0 -0.2
growth on ricinoleic acid YNB Glucose -0.2 +0.1 +0.7 -0.2 +0.3
auxotrophy Dropout Complete -0.2 +0.2 +0.5 +0.1 +0.2
auxotrophy YNB Glucose 75mM Methionine +0.2 +0.2 +0.7 +0.1 -0.3
auxotrophy YNB Glucose 75mM Arginine +0.2 +0.1 +0.5 +0.1 -0.1
auxotrophy YNB Glucose +0.3 +0.1 +0.7 +0.0 -0.1
ypd buoyancy 40 hrs High Buoyancy +0.6 +0.4 +0.2 -0.2 +0.1
growth on ricinoleic acid YNB Methylricinoleic Acid +0.2 +0.3 +0.7 -0.0 +0.2
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