Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 13079 and 13080 are separated by 232 nucleotides13080 and 13081 are separated by 334 nucleotides13081 and 13082 are separated by 124 nucleotides13082 and 13083 are separated by 115 nucleotides mRNA_4711: 13079 - KOG1001 Helicase-like transcription factor HLTF/DNA helicase RAD5, DEAD-box superfamily, at 826,547 to 830,720 13079 mRNA_4712: 13080 - HMMPfam-N-terminal C2 in EEIG1 and EHBP1 proteins-PF10358, at 830,953 to 834,055 13080 mRNA_4713: 13081 - HMMPfam-Fungal Zn(2)-Cys(6) binuclear cluster domain-PF00172,HMMPfam-Fungal specific transcription factor domain-PF04082,ProSitePatterns-Zn(2)-C6 fungal-type DNA-binding domain signature.-PS00463,ProSiteProfiles-Zn(2)-C6 fungal-type DNA-binding domain profile.-PS50048,SMART-GAL4-like Zn(II)2Cys6 (or C6 zinc) binuclear cluster DNA-binding domain-SM00066,SUPERFAMILY--SSF57701, at 834,390 to 838,234 13081 mRNA_4714: 13082 - K01183 E3.2.1.14 chitinase, at 838,359 to 840,435 13082 mRNA_4715: 13083 - KOG1730 Thioredoxin-like protein, at 840,551 to 841,550 13083
Group Condition mRNA_4711 mRNA_4712 mRNA_4713 mRNA_4714 mRNA_4715
13079 13080 13081 13082 13083
ypd buoyancy 40 hrs No Buoyancy Separation +0.3 -0.5 -0.5 -1.7 -0.5
facs on lipid accumulation Sorted No BODIPY Gate -0.0 +0.0 -0.3 -0.7 -0.0
facs on lipid accumulation Sorted Low BODIPY Gate +0.0 -0.2 -0.6 -0.2 +0.0
buoyancy on lipid accumulation 40 hrs Low Buoyancy -0.2 -0.1 -0.4 -0.0 -0.0
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation -0.2 -0.2 -0.1 -0.1 +0.1
growth on ricinoleic acid YNB Ricinoleic Acid +0.3 -0.3 -0.1 -0.6 +0.4
buoyancy on lipid accumulation 40 hrs Median Buoyancy -0.1 +0.0 +0.0 -0.4 +0.2
ypd buoyancy 40 hrs Low Buoyancy +0.3 +0.1 -0.4 -0.1 +0.1
buoyancy on lipid accumulation 40 hrs High Buoyancy -0.2 -0.1 +0.1 +0.1 +0.1
growth on oleic acid YNB Oleic Acid -0.1 -0.2 -0.1 +0.2 +0.2
auxotrophy Dropout Complete +0.2 +0.5 +0.1 +0.2 -0.9
facs on lipid accumulation Sorted High BODIPY Gate -0.0 -0.1 -0.0 +0.3 +0.1
auxotrophy YNB Glucose +0.1 +0.7 +0.0 -0.1 -0.4
ypd buoyancy 40 hrs Median Buoyancy +0.0 -0.0 +0.0 -0.0 +0.3
auxotrophy YNB Glucose 75mM Arginine +0.1 +0.5 +0.1 -0.1 -0.3
auxotrophy YNB Glucose 75mM Methionine +0.2 +0.7 +0.1 -0.3 -0.1
growth on ypd YPD -0.0 +0.4 -0.2 +0.3 +0.2
ypd buoyancy 40 hrs High Buoyancy +0.4 +0.2 -0.2 +0.1 +0.3
growth on oleic acid YNB Glucose -0.0 +0.4 -0.0 -0.2 +0.6
growth on ricinoleic acid YNB Glucose +0.1 +0.7 -0.2 +0.3 +0.2
growth on ricinoleic acid YNB Methylricinoleic Acid +0.3 +0.7 -0.0 +0.2 +0.1
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