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Fitness for 5 genes in
Rhodosporidium toruloides IFO0880
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Top 21 experiments (either direction), sorted by average fitness
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500 nt
13079 and 13080 are separated by 232 nucleotides
13080 and 13081 are separated by 334 nucleotides
13081 and 13082 are separated by 124 nucleotides
13082 and 13083 are separated by 115 nucleotides
mRNA_4711: 13079 - KOG1001 Helicase-like transcription factor HLTF/DNA helicase RAD5, DEAD-box superfamily, at 826,547 to 830,720
13079
mRNA_4712: 13080 - HMMPfam-N-terminal C2 in EEIG1 and EHBP1 proteins-PF10358, at 830,953 to 834,055
13080
mRNA_4713: 13081 - HMMPfam-Fungal Zn(2)-Cys(6) binuclear cluster domain-PF00172,HMMPfam-Fungal specific transcription factor domain-PF04082,ProSitePatterns-Zn(2)-C6 fungal-type DNA-binding domain signature.-PS00463,ProSiteProfiles-Zn(2)-C6 fungal-type DNA-binding domain profile.-PS50048,SMART-GAL4-like Zn(II)2Cys6 (or C6 zinc) binuclear cluster DNA-binding domain-SM00066,SUPERFAMILY--SSF57701, at 834,390 to 838,234
13081
mRNA_4714: 13082 - K01183 E3.2.1.14 chitinase, at 838,359 to 840,435
13082
mRNA_4715: 13083 - KOG1730 Thioredoxin-like protein, at 840,551 to 841,550
13083
Group
Condition
mRNA
_4711
mRNA
_4712
mRNA
_4713
mRNA
_4714
mRNA
_4715
13079
13080
13081
13082
13083
ypd buoyancy
40 hrs No Buoyancy Separation
+0.3
-0.5
-0.5
-1.7
-0.5
facs on lipid accumulation
Sorted No BODIPY Gate
-0.0
+0.0
-0.3
-0.7
-0.0
facs on lipid accumulation
Sorted Low BODIPY Gate
+0.0
-0.2
-0.6
-0.2
+0.0
buoyancy on lipid accumulation
40 hrs Low Buoyancy
-0.2
-0.1
-0.4
-0.0
-0.0
buoyancy on lipid accumulation
40 hrs No Buoyancy Separation
-0.2
-0.2
-0.1
-0.1
+0.1
growth on ricinoleic acid
YNB Ricinoleic Acid
+0.3
-0.3
-0.1
-0.6
+0.4
buoyancy on lipid accumulation
40 hrs Median Buoyancy
-0.1
+0.0
+0.0
-0.4
+0.2
ypd buoyancy
40 hrs Low Buoyancy
+0.3
+0.1
-0.4
-0.1
+0.1
buoyancy on lipid accumulation
40 hrs High Buoyancy
-0.2
-0.1
+0.1
+0.1
+0.1
growth on oleic acid
YNB Oleic Acid
-0.1
-0.2
-0.1
+0.2
+0.2
auxotrophy
Dropout Complete
+0.2
+0.5
+0.1
+0.2
-0.9
facs on lipid accumulation
Sorted High BODIPY Gate
-0.0
-0.1
-0.0
+0.3
+0.1
auxotrophy
YNB Glucose
+0.1
+0.7
+0.0
-0.1
-0.4
ypd buoyancy
40 hrs Median Buoyancy
+0.0
-0.0
+0.0
-0.0
+0.3
auxotrophy
YNB Glucose 75mM Arginine
+0.1
+0.5
+0.1
-0.1
-0.3
auxotrophy
YNB Glucose 75mM Methionine
+0.2
+0.7
+0.1
-0.3
-0.1
growth on ypd
YPD
-0.0
+0.4
-0.2
+0.3
+0.2
ypd buoyancy
40 hrs High Buoyancy
+0.4
+0.2
-0.2
+0.1
+0.3
growth on oleic acid
YNB Glucose
-0.0
+0.4
-0.0
-0.2
+0.6
growth on ricinoleic acid
YNB Glucose
+0.1
+0.7
-0.2
+0.3
+0.2
growth on ricinoleic acid
YNB Methylricinoleic Acid
+0.3
+0.7
-0.0
+0.2
+0.1
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