Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 13088 and 13089 are separated by 242 nucleotides13089 and 13090 are separated by 345 nucleotides13090 and 13091 are separated by 244 nucleotides13091 and 13092 are separated by 220 nucleotides mRNA_4720: 13088 - K01415 ECE endothelin-converting enzyme, at 851,220 to 854,422 13088 mRNA_4721: 13089 - K10643 CNOT4, NOT4, MOT2 CCR4-NOT transcription complex subunit 4, at 854,665 to 858,103 13089 mRNA_4722: 13090 - K01027 OXCT 3-oxoacid CoA-transferase, at 858,449 to 860,854 13090 mRNA_4723: 13091 - Hypothetical Protein, at 861,099 to 862,312 13091 mRNA_4724: 13092 - Hypothetical Protein, at 862,533 to 863,753 13092
Group Condition mRNA_4720 mRNA_4721 mRNA_4722 mRNA_4723 mRNA_4724
13088 13089 13090 13091 13092
facs on lipid accumulation Sorted Low BODIPY Gate -0.3 -2.8 +0.0 -1.8 +0.1
growth on ricinoleic acid YNB Ricinoleic Acid +0.1 -3.6 +0.0 -0.2 -0.1
facs on lipid accumulation Sorted High BODIPY Gate -0.2 -3.6 +0.1 -0.7 +0.8
ypd buoyancy 40 hrs No Buoyancy Separation -0.3 -0.1 -0.1 -1.8 -0.9
buoyancy on lipid accumulation 40 hrs Low Buoyancy -0.0 -0.5 -0.2 -1.4 -0.9
buoyancy on lipid accumulation 40 hrs Median Buoyancy +0.0 -1.7 +0.1 -0.8 -0.2
growth on ricinoleic acid YNB Glucose -0.3 -1.9 +0.0 -0.1 -0.1
facs on lipid accumulation Sorted No BODIPY Gate -0.1 -3.0 -0.1 +0.3 +0.5
auxotrophy Dropout Complete -0.1 -2.1 +0.1 +0.8 -0.7
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation -0.2 -0.3 -0.1 -0.6 -0.9
ypd buoyancy 40 hrs Low Buoyancy -0.1 -0.5 -0.3 -0.3 -0.4
auxotrophy YNB Glucose 75mM Arginine -0.1 -1.7 +0.0 +0.8 -0.2
buoyancy on lipid accumulation 40 hrs High Buoyancy +0.1 +0.2 +0.0 -0.5 -0.4
ypd buoyancy 40 hrs High Buoyancy -0.2 +0.6 -0.2 -0.4 -0.2
ypd buoyancy 40 hrs Median Buoyancy -0.1 +0.7 -0.2 -0.4 -0.3
auxotrophy YNB Glucose 75mM Methionine +0.1 -0.6 +0.1 +0.8 -0.6
growth on ypd YPD +0.2 -0.8 +0.1 +1.0 +0.2
auxotrophy YNB Glucose -0.2 +0.7 -0.0 +0.7 -0.4
growth on ricinoleic acid YNB Methylricinoleic Acid -0.1 +0.1 +0.2 +0.6 +0.1
growth on oleic acid YNB Oleic Acid -0.2 +1.8 +0.0 -0.1 +0.1
growth on oleic acid YNB Glucose +0.1 +0.3 +0.1 +1.0 +0.1
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