Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 13127 and 13128 are separated by 174 nucleotides13128 and 13129 are separated by 315 nucleotides13129 and 13130 are separated by 1095 nucleotides13130 and 13131 are separated by 709 nucleotides mRNA_4759: 13127 - HMMPfam-Fungal cellulose binding domain-PF00734,ProSiteProfiles-CBM1 (carbohydrate binding type-1) domain profile.-PS51164,SMART-Fungal-type cellulose-binding domain-SM00236,SUPERFAMILY--SSF57180, at 940,381 to 941,157 13127 mRNA_4760: 13128 - K08157 TPO1 MFS transporter, DHA1 family, multidrug resistance protein, at 941,332 to 944,070 13128 mRNA_4761: 13129 - K02735 PSMB3 20S proteasome subunit beta 3, at 944,386 to 945,694 13129 mRNA_4762: 13130 - HMMPfam-Fungal cellulose binding domain-PF00734,ProSitePatterns-CBM1 (carbohydrate binding type-1) domain signature.-PS00562,ProSiteProfiles-CBM1 (carbohydrate binding type-1) domain profile.-PS51164,SMART-Fungal-type cellulose-binding domain-SM00236,SUPERFAMILY--SSF57180, at 946,790 to 947,960 13130 mRNA_4763: 13131 - KOG1263 Multicopper oxidases, at 948,670 to 952,404 13131
Group Condition mRNA_4759 mRNA_4760 mRNA_4761 mRNA_4762 mRNA_4763
13127 13128 13129 13130 13131
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation -0.6 -0.1 N.D. -0.7 -0.3
growth on ricinoleic acid YNB Glucose +0.2 -0.4 N.D. -1.1 +0.1
ypd buoyancy 40 hrs No Buoyancy Separation +0.2 -0.3 N.D. -0.8 -0.1
facs on lipid accumulation Sorted Low BODIPY Gate +0.2 -0.2 N.D. -1.1 +0.1
ypd buoyancy 40 hrs High Buoyancy -0.3 +0.2 N.D. -0.5 -0.1
auxotrophy YNB Glucose 75mM Methionine +0.5 -0.2 N.D. -1.0 +0.0
buoyancy on lipid accumulation 40 hrs Median Buoyancy -0.4 -0.0 N.D. -0.1 +0.2
growth on ypd YPD +0.0 +0.0 N.D. +0.4 -0.5
auxotrophy Dropout Complete +0.0 -0.1 N.D. +0.1 -0.0
buoyancy on lipid accumulation 40 hrs Low Buoyancy -0.2 -0.0 N.D. +0.2 +0.1
facs on lipid accumulation Sorted High BODIPY Gate +0.2 -0.1 N.D. +0.2 -0.1
auxotrophy YNB Glucose 75mM Arginine +0.1 -0.1 N.D. +0.1 +0.1
auxotrophy YNB Glucose +0.5 -0.2 N.D. -0.1 +0.1
facs on lipid accumulation Sorted No BODIPY Gate -0.1 -0.2 N.D. +0.6 -0.0
growth on ricinoleic acid YNB Methylricinoleic Acid +0.5 +0.0 N.D. +0.1 -0.0
ypd buoyancy 40 hrs Low Buoyancy +0.4 +0.0 N.D. +0.3 -0.0
buoyancy on lipid accumulation 40 hrs High Buoyancy -0.2 +0.1 N.D. +0.7 +0.2
ypd buoyancy 40 hrs Median Buoyancy +0.2 +0.3 N.D. +0.2 +0.1
growth on ricinoleic acid YNB Ricinoleic Acid +0.5 -0.1 N.D. +0.6 +0.1
growth on oleic acid YNB Oleic Acid +0.6 +0.3 N.D. +0.4 -0.0
growth on oleic acid YNB Glucose +0.2 -0.0 N.D. +1.1 +0.2
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