Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 13129 and 13130 are separated by 1095 nucleotides13130 and 13131 are separated by 709 nucleotides13131 and 13132 are separated by 2243 nucleotides13132 and 13133 are separated by 279 nucleotides mRNA_4761: 13129 - K02735 PSMB3 20S proteasome subunit beta 3, at 944,386 to 945,694 13129 mRNA_4762: 13130 - HMMPfam-Fungal cellulose binding domain-PF00734,ProSitePatterns-CBM1 (carbohydrate binding type-1) domain signature.-PS00562,ProSiteProfiles-CBM1 (carbohydrate binding type-1) domain profile.-PS51164,SMART-Fungal-type cellulose-binding domain-SM00236,SUPERFAMILY--SSF57180, at 946,790 to 947,960 13130 mRNA_4763: 13131 - KOG1263 Multicopper oxidases, at 948,670 to 952,404 13131 mRNA_4764: 13132 - K12842 SR140 U2-associated protein SR140, at 954,648 to 957,720 13132 mRNA_4765: 13133 - SUPERFAMILY--SSF52058, at 958,000 to 961,527 13133
Group Condition mRNA_4761 mRNA_4762 mRNA_4763 mRNA_4764 mRNA_4765
13129 13130 13131 13132 13133
facs on lipid accumulation Sorted Low BODIPY Gate N.D. -1.1 +0.1 -1.9 -0.1
auxotrophy YNB Glucose 75mM Methionine N.D. -1.0 +0.0 -1.4 -0.2
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation N.D. -0.7 -0.3 -1.1 -0.3
facs on lipid accumulation Sorted High BODIPY Gate N.D. +0.2 -0.1 -2.4 +0.1
ypd buoyancy 40 hrs No Buoyancy Separation N.D. -0.8 -0.1 -1.2 +0.2
auxotrophy YNB Glucose N.D. -0.1 +0.1 -1.5 -0.2
facs on lipid accumulation Sorted No BODIPY Gate N.D. +0.6 -0.0 -2.4 +0.1
auxotrophy YNB Glucose 75mM Arginine N.D. +0.1 +0.1 -1.5 -0.1
growth on ricinoleic acid YNB Glucose N.D. -1.1 +0.1 -0.4 -0.0
growth on ricinoleic acid YNB Ricinoleic Acid N.D. +0.6 +0.1 -1.7 -0.0
ypd buoyancy 40 hrs High Buoyancy N.D. -0.5 -0.1 -0.4 +0.1
auxotrophy Dropout Complete N.D. +0.1 -0.0 -0.2 -0.2
buoyancy on lipid accumulation 40 hrs Low Buoyancy N.D. +0.2 +0.1 -0.5 -0.0
growth on oleic acid YNB Oleic Acid N.D. +0.4 -0.0 -0.4 -0.1
growth on oleic acid YNB Glucose N.D. +1.1 +0.2 -1.2 -0.1
growth on ricinoleic acid YNB Methylricinoleic Acid N.D. +0.1 -0.0 -0.2 +0.1
growth on ypd YPD N.D. +0.4 -0.5 +0.5 -0.1
buoyancy on lipid accumulation 40 hrs Median Buoyancy N.D. -0.1 +0.2 +0.9 +0.1
ypd buoyancy 40 hrs Low Buoyancy N.D. +0.3 -0.0 +0.9 +0.0
buoyancy on lipid accumulation 40 hrs High Buoyancy N.D. +0.7 +0.2 +0.7 -0.1
ypd buoyancy 40 hrs Median Buoyancy N.D. +0.2 +0.1 +1.5 +0.0
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