Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 13137 and 13138 are separated by 374 nucleotides13138 and 13139 are separated by 921 nucleotides13139 and 13140 are separated by 358 nucleotides13140 and 13141 are separated by 340 nucleotides mRNA_4769: 13137 - K01866 YARS, tyrS tyrosyl-tRNA synthetase, at 969,625 to 971,504 13137 mRNA_4770: 13138 - KOG0988 RNA-directed RNA polymerase QDE-1 required for posttranscriptional gene silencing and RNA interference, at 971,879 to 977,050 13138 mRNA_4771: 13139 - K20062 ART4_5_7 arrestin-related trafficking adapter 4/5/7, at 977,972 to 980,923 13139 mRNA_4772: 13140 - K01874 MARS, metG methionyl-tRNA synthetase, at 981,282 to 984,075 13140 mRNA_4773: 13141 - KOG0254 Predicted transporter (major facilitator superfamily), at 984,416 to 986,874 13141
Group Condition mRNA_4769 mRNA_4770 mRNA_4771 mRNA_4772 mRNA_4773
13137 13138 13139 13140 13141
ypd buoyancy 40 hrs No Buoyancy Separation N.D. -0.4 N.D. N.D. -1.3
ypd buoyancy 40 hrs High Buoyancy N.D. -0.1 N.D. N.D. -0.8
ypd buoyancy 40 hrs Low Buoyancy N.D. -0.1 N.D. N.D. -0.8
facs on lipid accumulation Sorted Low BODIPY Gate N.D. -0.0 N.D. N.D. -0.5
buoyancy on lipid accumulation 40 hrs High Buoyancy N.D. +0.2 N.D. N.D. -0.8
ypd buoyancy 40 hrs Median Buoyancy N.D. -0.1 N.D. N.D. -0.4
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation N.D. +0.1 N.D. N.D. -0.4
buoyancy on lipid accumulation 40 hrs Median Buoyancy N.D. +0.1 N.D. N.D. -0.4
growth on ricinoleic acid YNB Glucose N.D. +0.1 N.D. N.D. -0.2
growth on ypd YPD N.D. -0.1 N.D. N.D. -0.0
buoyancy on lipid accumulation 40 hrs Low Buoyancy N.D. +0.1 N.D. N.D. -0.1
growth on ricinoleic acid YNB Methylricinoleic Acid N.D. +0.1 N.D. N.D. -0.1
facs on lipid accumulation Sorted No BODIPY Gate N.D. +0.1 N.D. N.D. -0.1
growth on ricinoleic acid YNB Ricinoleic Acid N.D. +0.2 N.D. N.D. -0.2
auxotrophy YNB Glucose 75mM Methionine N.D. +0.0 N.D. N.D. +0.0
auxotrophy YNB Glucose 75mM Arginine N.D. +0.0 N.D. N.D. +0.1
auxotrophy Dropout Complete N.D. -0.0 N.D. N.D. +0.3
auxotrophy YNB Glucose N.D. -0.1 N.D. N.D. +0.4
facs on lipid accumulation Sorted High BODIPY Gate N.D. +0.1 N.D. N.D. +0.3
growth on oleic acid YNB Glucose N.D. -0.0 N.D. N.D. +0.4
growth on oleic acid YNB Oleic Acid N.D. -0.1 N.D. N.D. +0.8
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