Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 13138 and 13139 are separated by 921 nucleotides13139 and 13140 are separated by 358 nucleotides13140 and 13141 are separated by 340 nucleotides13141 and 13142 are separated by 704 nucleotides mRNA_4770: 13138 - KOG0988 RNA-directed RNA polymerase QDE-1 required for posttranscriptional gene silencing and RNA interference, at 971,879 to 977,050 13138 mRNA_4771: 13139 - K20062 ART4_5_7 arrestin-related trafficking adapter 4/5/7, at 977,972 to 980,923 13139 mRNA_4772: 13140 - K01874 MARS, metG methionyl-tRNA synthetase, at 981,282 to 984,075 13140 mRNA_4773: 13141 - KOG0254 Predicted transporter (major facilitator superfamily), at 984,416 to 986,874 13141 mRNA_4774: 13142 - K02998 RP-SAe, RPSA small subunit ribosomal protein SAe, at 987,579 to 988,756 13142
Group Condition mRNA_4770 mRNA_4771 mRNA_4772 mRNA_4773 mRNA_4774
13138 13139 13140 13141 13142
ypd buoyancy 40 hrs No Buoyancy Separation -0.4 N.D. N.D. -1.3 N.D.
ypd buoyancy 40 hrs High Buoyancy -0.1 N.D. N.D. -0.8 N.D.
ypd buoyancy 40 hrs Low Buoyancy -0.1 N.D. N.D. -0.8 N.D.
facs on lipid accumulation Sorted Low BODIPY Gate -0.0 N.D. N.D. -0.5 N.D.
buoyancy on lipid accumulation 40 hrs High Buoyancy +0.2 N.D. N.D. -0.8 N.D.
ypd buoyancy 40 hrs Median Buoyancy -0.1 N.D. N.D. -0.4 N.D.
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation +0.1 N.D. N.D. -0.4 N.D.
buoyancy on lipid accumulation 40 hrs Median Buoyancy +0.1 N.D. N.D. -0.4 N.D.
growth on ricinoleic acid YNB Glucose +0.1 N.D. N.D. -0.2 N.D.
growth on ypd YPD -0.1 N.D. N.D. -0.0 N.D.
buoyancy on lipid accumulation 40 hrs Low Buoyancy +0.1 N.D. N.D. -0.1 N.D.
growth on ricinoleic acid YNB Methylricinoleic Acid +0.1 N.D. N.D. -0.1 N.D.
facs on lipid accumulation Sorted No BODIPY Gate +0.1 N.D. N.D. -0.1 N.D.
growth on ricinoleic acid YNB Ricinoleic Acid +0.2 N.D. N.D. -0.2 N.D.
auxotrophy YNB Glucose 75mM Methionine +0.0 N.D. N.D. +0.0 N.D.
auxotrophy YNB Glucose 75mM Arginine +0.0 N.D. N.D. +0.1 N.D.
auxotrophy Dropout Complete -0.0 N.D. N.D. +0.3 N.D.
auxotrophy YNB Glucose -0.1 N.D. N.D. +0.4 N.D.
facs on lipid accumulation Sorted High BODIPY Gate +0.1 N.D. N.D. +0.3 N.D.
growth on oleic acid YNB Glucose -0.0 N.D. N.D. +0.4 N.D.
growth on oleic acid YNB Oleic Acid -0.1 N.D. N.D. +0.8 N.D.
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