Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 13139 and 13140 are separated by 358 nucleotides13140 and 13141 are separated by 340 nucleotides13141 and 13142 are separated by 704 nucleotides13142 and 13143 are separated by 629 nucleotides mRNA_4771: 13139 - K20062 ART4_5_7 arrestin-related trafficking adapter 4/5/7, at 977,972 to 980,923 13139 mRNA_4772: 13140 - K01874 MARS, metG methionyl-tRNA synthetase, at 981,282 to 984,075 13140 mRNA_4773: 13141 - KOG0254 Predicted transporter (major facilitator superfamily), at 984,416 to 986,874 13141 mRNA_4774: 13142 - K02998 RP-SAe, RPSA small subunit ribosomal protein SAe, at 987,579 to 988,756 13142 mRNA_4775: 13143 - K01724 PCBD, phhB 4a-hydroxytetrahydrobiopterin dehydratase, at 989,386 to 989,809 13143
Group Condition mRNA_4771 mRNA_4772 mRNA_4773 mRNA_4774 mRNA_4775
13139 13140 13141 13142 13143
ypd buoyancy 40 hrs No Buoyancy Separation N.D. N.D. -1.3 N.D. -0.3
ypd buoyancy 40 hrs Low Buoyancy N.D. N.D. -0.8 N.D. +0.0
buoyancy on lipid accumulation 40 hrs High Buoyancy N.D. N.D. -0.8 N.D. +0.1
facs on lipid accumulation Sorted No BODIPY Gate N.D. N.D. -0.1 N.D. -0.6
buoyancy on lipid accumulation 40 hrs Median Buoyancy N.D. N.D. -0.4 N.D. -0.2
growth on ypd YPD N.D. N.D. -0.0 N.D. -0.5
ypd buoyancy 40 hrs High Buoyancy N.D. N.D. -0.8 N.D. +0.5
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation N.D. N.D. -0.4 N.D. +0.1
buoyancy on lipid accumulation 40 hrs Low Buoyancy N.D. N.D. -0.1 N.D. -0.2
ypd buoyancy 40 hrs Median Buoyancy N.D. N.D. -0.4 N.D. +0.1
auxotrophy YNB Glucose 75mM Methionine N.D. N.D. +0.0 N.D. -0.3
growth on ricinoleic acid YNB Methylricinoleic Acid N.D. N.D. -0.1 N.D. -0.1
facs on lipid accumulation Sorted Low BODIPY Gate N.D. N.D. -0.5 N.D. +0.5
growth on ricinoleic acid YNB Glucose N.D. N.D. -0.2 N.D. +0.2
growth on ricinoleic acid YNB Ricinoleic Acid N.D. N.D. -0.2 N.D. +0.2
facs on lipid accumulation Sorted High BODIPY Gate N.D. N.D. +0.3 N.D. -0.3
auxotrophy YNB Glucose 75mM Arginine N.D. N.D. +0.1 N.D. -0.0
auxotrophy Dropout Complete N.D. N.D. +0.3 N.D. -0.0
auxotrophy YNB Glucose N.D. N.D. +0.4 N.D. +0.1
growth on oleic acid YNB Glucose N.D. N.D. +0.4 N.D. +0.2
growth on oleic acid YNB Oleic Acid N.D. N.D. +0.8 N.D. -0.1
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