Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 13200 and 13201 are separated by 190 nucleotides13201 and 13202 are separated by 295 nucleotides13202 and 13203 are separated by 934 nucleotides13203 and 13204 are separated by 310 nucleotides mRNA_4832: 13200 - KOG4168 Predicted RNA polymerase III subunit C17, at 1,109,759 to 1,110,628 13200 mRNA_4833: 13201 - K05755 ARPC4 actin related protein 2/3 complex, subunit 4, at 1,110,819 to 1,111,714 13201 mRNA_4834: 13202 - K02996 RP-S9, MRPS9, rpsI small subunit ribosomal protein S9, at 1,112,010 to 1,113,278 13202 mRNA_4835: 13203 - KOG0490 Transcription factor, contains HOX domain, at 1,114,213 to 1,116,199 13203 mRNA_4836: 13204 - BLAST NEK protein kinase [Microbotryum lychnidis-dioicae p1..., at 1,116,510 to 1,117,726 13204
Group Condition mRNA_4832 mRNA_4833 mRNA_4834 mRNA_4835 mRNA_4836
13200 13201 13202 13203 13204
ypd buoyancy 40 hrs No Buoyancy Separation N.D. N.D. N.D. +0.1 -1.9
buoyancy on lipid accumulation 40 hrs Low Buoyancy N.D. N.D. N.D. -0.9 -0.5
facs on lipid accumulation Sorted High BODIPY Gate N.D. N.D. N.D. -0.3 -0.4
growth on ypd YPD N.D. N.D. N.D. +0.1 -0.7
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation N.D. N.D. N.D. -0.6 +0.0
growth on ricinoleic acid YNB Ricinoleic Acid N.D. N.D. N.D. +0.1 -0.6
buoyancy on lipid accumulation 40 hrs High Buoyancy N.D. N.D. N.D. -0.8 +0.3
growth on ricinoleic acid YNB Glucose N.D. N.D. N.D. -0.1 -0.2
auxotrophy YNB Glucose N.D. N.D. N.D. -0.2 -0.1
buoyancy on lipid accumulation 40 hrs Median Buoyancy N.D. N.D. N.D. -0.4 +0.2
growth on oleic acid YNB Oleic Acid N.D. N.D. N.D. -0.2 +0.0
growth on oleic acid YNB Glucose N.D. N.D. N.D. -0.2 +0.1
facs on lipid accumulation Sorted No BODIPY Gate N.D. N.D. N.D. -0.1 +0.1
auxotrophy YNB Glucose 75mM Methionine N.D. N.D. N.D. +0.0 +0.2
auxotrophy YNB Glucose 75mM Arginine N.D. N.D. N.D. -0.1 +0.4
growth on ricinoleic acid YNB Methylricinoleic Acid N.D. N.D. N.D. +0.4 -0.1
ypd buoyancy 40 hrs Low Buoyancy N.D. N.D. N.D. +0.3 +0.2
facs on lipid accumulation Sorted Low BODIPY Gate N.D. N.D. N.D. -0.1 +0.8
auxotrophy Dropout Complete N.D. N.D. N.D. +0.1 +0.6
ypd buoyancy 40 hrs High Buoyancy N.D. N.D. N.D. +0.3 +0.6
ypd buoyancy 40 hrs Median Buoyancy N.D. N.D. N.D. +0.4 +0.5
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