Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 13202 and 13203 are separated by 934 nucleotides13203 and 13204 are separated by 310 nucleotides13204 and 13205 are separated by 16 nucleotides13205 and 13206 are separated by 1519 nucleotides mRNA_4834: 13202 - K02996 RP-S9, MRPS9, rpsI small subunit ribosomal protein S9, at 1,112,010 to 1,113,278 13202 mRNA_4835: 13203 - KOG0490 Transcription factor, contains HOX domain, at 1,114,213 to 1,116,199 13203 mRNA_4836: 13204 - BLAST NEK protein kinase [Microbotryum lychnidis-dioicae p1..., at 1,116,510 to 1,117,726 13204 mRNA_4837: 13205 - K20872 NEK2 NIMA (never in mitosis gene a)-related kinase 2, at 1,117,743 to 1,117,911 13205 mRNA_4838: 13206 - K00326 E1.6.2.2 cytochrome-b5 reductase, at 1,119,431 to 1,120,749 13206
Group Condition mRNA_4834 mRNA_4835 mRNA_4836 mRNA_4837 mRNA_4838
13202 13203 13204 13205 13206
ypd buoyancy 40 hrs No Buoyancy Separation N.D. +0.1 -1.9 N.D. N.D.
buoyancy on lipid accumulation 40 hrs Low Buoyancy N.D. -0.9 -0.5 N.D. N.D.
facs on lipid accumulation Sorted High BODIPY Gate N.D. -0.3 -0.4 N.D. N.D.
growth on ypd YPD N.D. +0.1 -0.7 N.D. N.D.
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation N.D. -0.6 +0.0 N.D. N.D.
growth on ricinoleic acid YNB Ricinoleic Acid N.D. +0.1 -0.6 N.D. N.D.
buoyancy on lipid accumulation 40 hrs High Buoyancy N.D. -0.8 +0.3 N.D. N.D.
growth on ricinoleic acid YNB Glucose N.D. -0.1 -0.2 N.D. N.D.
auxotrophy YNB Glucose N.D. -0.2 -0.1 N.D. N.D.
buoyancy on lipid accumulation 40 hrs Median Buoyancy N.D. -0.4 +0.2 N.D. N.D.
growth on oleic acid YNB Oleic Acid N.D. -0.2 +0.0 N.D. N.D.
growth on oleic acid YNB Glucose N.D. -0.2 +0.1 N.D. N.D.
facs on lipid accumulation Sorted No BODIPY Gate N.D. -0.1 +0.1 N.D. N.D.
auxotrophy YNB Glucose 75mM Methionine N.D. +0.0 +0.2 N.D. N.D.
auxotrophy YNB Glucose 75mM Arginine N.D. -0.1 +0.4 N.D. N.D.
growth on ricinoleic acid YNB Methylricinoleic Acid N.D. +0.4 -0.1 N.D. N.D.
ypd buoyancy 40 hrs Low Buoyancy N.D. +0.3 +0.2 N.D. N.D.
facs on lipid accumulation Sorted Low BODIPY Gate N.D. -0.1 +0.8 N.D. N.D.
auxotrophy Dropout Complete N.D. +0.1 +0.6 N.D. N.D.
ypd buoyancy 40 hrs High Buoyancy N.D. +0.3 +0.6 N.D. N.D.
ypd buoyancy 40 hrs Median Buoyancy N.D. +0.4 +0.5 N.D. N.D.
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