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Fitness for 5 genes in
Rhodosporidium toruloides IFO0880
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Top 21 experiments (either direction), sorted by average fitness
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500 nt
13203 and 13204 are separated by 310 nucleotides
13204 and 13205 are separated by 16 nucleotides
13205 and 13206 are separated by 1519 nucleotides
13206 and 13207 are separated by 254 nucleotides
mRNA_4835: 13203 - KOG0490 Transcription factor, contains HOX domain, at 1,114,213 to 1,116,199
13203
mRNA_4836: 13204 - BLAST NEK protein kinase [Microbotryum lychnidis-dioicae p1..., at 1,116,510 to 1,117,726
13204
mRNA_4837: 13205 - K20872 NEK2 NIMA (never in mitosis gene a)-related kinase 2, at 1,117,743 to 1,117,911
13205
mRNA_4838: 13206 - K00326 E1.6.2.2 cytochrome-b5 reductase, at 1,119,431 to 1,120,749
13206
mRNA_4839: 13207 - K20300 TRAPPC1, BET5 trafficking protein particle complex subunit 1, at 1,121,004 to 1,121,635
13207
Group
Condition
mRNA
_4835
mRNA
_4836
mRNA
_4837
mRNA
_4838
mRNA
_4839
13203
13204
13205
13206
13207
ypd buoyancy
40 hrs No Buoyancy Separation
+0.1
-1.9
N.D.
N.D.
N.D.
buoyancy on lipid accumulation
40 hrs Low Buoyancy
-0.9
-0.5
N.D.
N.D.
N.D.
facs on lipid accumulation
Sorted High BODIPY Gate
-0.3
-0.4
N.D.
N.D.
N.D.
growth on ypd
YPD
+0.1
-0.7
N.D.
N.D.
N.D.
buoyancy on lipid accumulation
40 hrs No Buoyancy Separation
-0.6
+0.0
N.D.
N.D.
N.D.
growth on ricinoleic acid
YNB Ricinoleic Acid
+0.1
-0.6
N.D.
N.D.
N.D.
buoyancy on lipid accumulation
40 hrs High Buoyancy
-0.8
+0.3
N.D.
N.D.
N.D.
growth on ricinoleic acid
YNB Glucose
-0.1
-0.2
N.D.
N.D.
N.D.
auxotrophy
YNB Glucose
-0.2
-0.1
N.D.
N.D.
N.D.
buoyancy on lipid accumulation
40 hrs Median Buoyancy
-0.4
+0.2
N.D.
N.D.
N.D.
growth on oleic acid
YNB Oleic Acid
-0.2
+0.0
N.D.
N.D.
N.D.
growth on oleic acid
YNB Glucose
-0.2
+0.1
N.D.
N.D.
N.D.
facs on lipid accumulation
Sorted No BODIPY Gate
-0.1
+0.1
N.D.
N.D.
N.D.
auxotrophy
YNB Glucose 75mM Methionine
+0.0
+0.2
N.D.
N.D.
N.D.
auxotrophy
YNB Glucose 75mM Arginine
-0.1
+0.4
N.D.
N.D.
N.D.
growth on ricinoleic acid
YNB Methylricinoleic Acid
+0.4
-0.1
N.D.
N.D.
N.D.
ypd buoyancy
40 hrs Low Buoyancy
+0.3
+0.2
N.D.
N.D.
N.D.
facs on lipid accumulation
Sorted Low BODIPY Gate
-0.1
+0.8
N.D.
N.D.
N.D.
auxotrophy
Dropout Complete
+0.1
+0.6
N.D.
N.D.
N.D.
ypd buoyancy
40 hrs High Buoyancy
+0.3
+0.6
N.D.
N.D.
N.D.
ypd buoyancy
40 hrs Median Buoyancy
+0.4
+0.5
N.D.
N.D.
N.D.
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