Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 13214 and 13215 are separated by 253 nucleotides13215 and 13216 are separated by 50 nucleotides13216 and 13217 are separated by 192 nucleotides13217 and 13218 are separated by 347 nucleotides mRNA_4846: 13214 - K03943 NDUFV2 NADH dehydrogenase (ubiquinone) flavoprotein 2, at 1,132,189 to 1,133,318 13214 mRNA_4847: 13215 - Hypothetical Protein, at 1,133,572 to 1,137,413 13215 mRNA_4848: 13216 - Hypothetical Protein, at 1,137,464 to 1,141,122 13216 mRNA_4849: 13217 - HMMPfam-FAD binding domain-PF01565,HMMPfam-Berberine and berberine like-PF08031,ProSitePatterns-Oxygen oxidoreductases covalent FAD-binding site.-PS00862,ProSiteProfiles-PCMH-type FAD-binding domain profile.-PS51387,SUPERFAMILY--SSF56176, at 1,141,315 to 1,143,566 13217 mRNA_4850: 13218 - K02309 DBF4 regulatory subunit for Cdc7p protein kinase, at 1,143,914 to 1,146,258 13218
Group Condition mRNA_4846 mRNA_4847 mRNA_4848 mRNA_4849 mRNA_4850
13214 13215 13216 13217 13218
ypd buoyancy 40 hrs No Buoyancy Separation N.D. -0.2 -0.3 -0.5 N.D.
ypd buoyancy 40 hrs Low Buoyancy N.D. -0.0 -0.2 -0.3 N.D.
ypd buoyancy 40 hrs High Buoyancy N.D. -0.1 -0.1 -0.3 N.D.
facs on lipid accumulation Sorted Low BODIPY Gate N.D. +0.1 +0.0 -0.5 N.D.
facs on lipid accumulation Sorted No BODIPY Gate N.D. +0.0 -0.1 -0.3 N.D.
ypd buoyancy 40 hrs Median Buoyancy N.D. +0.1 -0.1 -0.2 N.D.
facs on lipid accumulation Sorted High BODIPY Gate N.D. -0.2 -0.2 +0.1 N.D.
auxotrophy Dropout Complete N.D. +0.1 -0.1 -0.2 N.D.
auxotrophy YNB Glucose N.D. +0.1 +0.1 -0.2 N.D.
growth on oleic acid YNB Glucose N.D. +0.0 -0.1 +0.1 N.D.
buoyancy on lipid accumulation 40 hrs High Buoyancy N.D. +0.1 +0.0 -0.1 N.D.
auxotrophy YNB Glucose 75mM Arginine N.D. +0.0 -0.0 +0.1 N.D.
auxotrophy YNB Glucose 75mM Methionine N.D. +0.1 +0.1 +0.0 N.D.
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation N.D. +0.1 +0.0 +0.1 N.D.
growth on ypd YPD N.D. +0.0 +0.1 +0.1 N.D.
growth on ricinoleic acid YNB Ricinoleic Acid N.D. +0.1 +0.2 +0.0 N.D.
buoyancy on lipid accumulation 40 hrs Low Buoyancy N.D. +0.1 -0.0 +0.2 N.D.
growth on oleic acid YNB Oleic Acid N.D. +0.0 +0.1 +0.3 N.D.
growth on ricinoleic acid YNB Glucose N.D. +0.0 +0.1 +0.3 N.D.
growth on ricinoleic acid YNB Methylricinoleic Acid N.D. +0.1 +0.1 +0.2 N.D.
buoyancy on lipid accumulation 40 hrs Median Buoyancy N.D. +0.1 +0.1 +0.3 N.D.
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