Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 13215 and 13216 are separated by 50 nucleotides13216 and 13217 are separated by 192 nucleotides13217 and 13218 are separated by 347 nucleotides13218 and 13219 are separated by 288 nucleotides mRNA_4847: 13215 - Hypothetical Protein, at 1,133,572 to 1,137,413 13215 mRNA_4848: 13216 - Hypothetical Protein, at 1,137,464 to 1,141,122 13216 mRNA_4849: 13217 - HMMPfam-FAD binding domain-PF01565,HMMPfam-Berberine and berberine like-PF08031,ProSitePatterns-Oxygen oxidoreductases covalent FAD-binding site.-PS00862,ProSiteProfiles-PCMH-type FAD-binding domain profile.-PS51387,SUPERFAMILY--SSF56176, at 1,141,315 to 1,143,566 13217 mRNA_4850: 13218 - K02309 DBF4 regulatory subunit for Cdc7p protein kinase, at 1,143,914 to 1,146,258 13218 mRNA_4851: 13219 - K06679 MAD1 mitotic spindle assembly checkpoint protein MAD1, at 1,146,547 to 1,149,479 13219
Group Condition mRNA_4847 mRNA_4848 mRNA_4849 mRNA_4850 mRNA_4851
13215 13216 13217 13218 13219
facs on lipid accumulation Sorted High BODIPY Gate -0.2 -0.2 +0.1 N.D. -0.7
facs on lipid accumulation Sorted Low BODIPY Gate +0.1 +0.0 -0.5 N.D. -0.2
ypd buoyancy 40 hrs Low Buoyancy -0.0 -0.2 -0.3 N.D. +0.0
facs on lipid accumulation Sorted No BODIPY Gate +0.0 -0.1 -0.3 N.D. -0.1
ypd buoyancy 40 hrs High Buoyancy -0.1 -0.1 -0.3 N.D. +0.2
auxotrophy Dropout Complete +0.1 -0.1 -0.2 N.D. -0.1
auxotrophy YNB Glucose +0.1 +0.1 -0.2 N.D. -0.1
growth on ypd YPD +0.0 +0.1 +0.1 N.D. -0.3
auxotrophy YNB Glucose 75mM Arginine +0.0 -0.0 +0.1 N.D. -0.1
ypd buoyancy 40 hrs No Buoyancy Separation -0.2 -0.3 -0.5 N.D. +1.0
auxotrophy YNB Glucose 75mM Methionine +0.1 +0.1 +0.0 N.D. -0.2
ypd buoyancy 40 hrs Median Buoyancy +0.1 -0.1 -0.2 N.D. +0.3
growth on oleic acid YNB Glucose +0.0 -0.1 +0.1 N.D. +0.0
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation +0.1 +0.0 +0.1 N.D. -0.1
growth on ricinoleic acid YNB Ricinoleic Acid +0.1 +0.2 +0.0 N.D. +0.1
growth on oleic acid YNB Oleic Acid +0.0 +0.1 +0.3 N.D. +0.1
buoyancy on lipid accumulation 40 hrs High Buoyancy +0.1 +0.0 -0.1 N.D. +0.4
growth on ricinoleic acid YNB Glucose +0.0 +0.1 +0.3 N.D. +0.1
buoyancy on lipid accumulation 40 hrs Median Buoyancy +0.1 +0.1 +0.3 N.D. +0.1
buoyancy on lipid accumulation 40 hrs Low Buoyancy +0.1 -0.0 +0.2 N.D. +0.4
growth on ricinoleic acid YNB Methylricinoleic Acid +0.1 +0.1 +0.2 N.D. +0.6
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