Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 13218 and 13219 are separated by 288 nucleotides13219 and 13220 are separated by 156 nucleotides13220 and 13221 are separated by 261 nucleotides13221 and 13222 are separated by 545 nucleotides mRNA_4850: 13218 - K02309 DBF4 regulatory subunit for Cdc7p protein kinase, at 1,143,914 to 1,146,258 13218 mRNA_4851: 13219 - K06679 MAD1 mitotic spindle assembly checkpoint protein MAD1, at 1,146,547 to 1,149,479 13219 mRNA_4852: 13220 - Hypothetical Protein, at 1,149,636 to 1,152,739 13220 mRNA_4853: 13221 - Hypothetical Protein, at 1,153,001 to 1,154,455 13221 mRNA_4854: 13222 - ProSiteProfiles-BTB domain profile.-PS50097, at 1,155,001 to 1,156,417 13222
Group Condition mRNA_4850 mRNA_4851 mRNA_4852 mRNA_4853 mRNA_4854
13218 13219 13220 13221 13222
facs on lipid accumulation Sorted High BODIPY Gate N.D. -0.7 -0.3 -0.9 +0.2
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation N.D. -0.1 +0.1 +0.4 -0.7
buoyancy on lipid accumulation 40 hrs Low Buoyancy N.D. +0.4 -0.1 +0.0 -0.3
auxotrophy YNB Glucose 75mM Methionine N.D. -0.2 -0.3 -0.1 +0.6
ypd buoyancy 40 hrs Low Buoyancy N.D. +0.0 -0.2 -0.1 +0.4
buoyancy on lipid accumulation 40 hrs High Buoyancy N.D. +0.4 -0.3 +0.4 -0.5
facs on lipid accumulation Sorted No BODIPY Gate N.D. -0.1 -0.1 +0.3 +0.1
facs on lipid accumulation Sorted Low BODIPY Gate N.D. -0.2 +0.1 +0.1 +0.2
buoyancy on lipid accumulation 40 hrs Median Buoyancy N.D. +0.1 +0.1 +0.3 -0.1
ypd buoyancy 40 hrs High Buoyancy N.D. +0.2 +0.0 -0.2 +0.5
growth on ypd YPD N.D. -0.3 +0.1 +0.9 -0.2
auxotrophy Dropout Complete N.D. -0.1 -0.0 -0.0 +0.7
auxotrophy YNB Glucose N.D. -0.1 -0.1 -0.1 +0.9
growth on oleic acid YNB Glucose N.D. +0.0 -0.1 +0.3 +0.4
growth on oleic acid YNB Oleic Acid N.D. +0.1 -0.0 +0.2 +0.6
auxotrophy YNB Glucose 75mM Arginine N.D. -0.1 +0.1 +0.1 +0.8
growth on ricinoleic acid YNB Ricinoleic Acid N.D. +0.1 +0.0 +0.3 +0.8
ypd buoyancy 40 hrs Median Buoyancy N.D. +0.3 +0.2 +0.4 +0.3
growth on ricinoleic acid YNB Glucose N.D. +0.1 -0.3 +0.6 +0.8
growth on ricinoleic acid YNB Methylricinoleic Acid N.D. +0.6 -0.1 +0.4 +0.7
ypd buoyancy 40 hrs No Buoyancy Separation N.D. +1.0 +0.2 -0.0 +0.5
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