Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 13229 and 13230 are separated by 112 nucleotides13230 and 13231 are separated by 217 nucleotides13231 and 13232 are separated by 146 nucleotides13232 and 13233 are separated by 824 nucleotides mRNA_4861: 13229 - BLAST NADH-ubiquinone oxidoreductase [Sanghuangporus baumii], at 1,165,741 to 1,167,081 13229 mRNA_4862: 13230 - K00817 hisC histidinol-phosphate aminotransferase, at 1,167,194 to 1,168,610 13230 mRNA_4863: 13231 - KOG0645 WD40 repeat protein, at 1,168,828 to 1,170,396 13231 mRNA_4864: 13232 - KOG0603 Ribosomal protein S6 kinase, at 1,170,543 to 1,172,403 13232 mRNA_4865: 13233 - K12860 CDC5L, CDC5, CEF1 pre-mRNA-splicing factor CDC5/CEF1, at 1,173,228 to 1,176,152 13233
Group Condition mRNA_4861 mRNA_4862 mRNA_4863 mRNA_4864 mRNA_4865
13229 13230 13231 13232 13233
ypd buoyancy 40 hrs No Buoyancy Separation -0.8 N.D. N.D. -0.5 N.D.
buoyancy on lipid accumulation 40 hrs High Buoyancy -0.8 N.D. N.D. -0.2 N.D.
growth on ypd YPD -0.2 N.D. N.D. -0.4 N.D.
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation -1.0 N.D. N.D. +0.5 N.D.
auxotrophy Dropout Complete -0.6 N.D. N.D. +0.2 N.D.
facs on lipid accumulation Sorted Low BODIPY Gate -0.7 N.D. N.D. +0.4 N.D.
ypd buoyancy 40 hrs Low Buoyancy -0.0 N.D. N.D. -0.2 N.D.
growth on ricinoleic acid YNB Methylricinoleic Acid -0.5 N.D. N.D. +0.4 N.D.
facs on lipid accumulation Sorted High BODIPY Gate -0.3 N.D. N.D. +0.2 N.D.
auxotrophy YNB Glucose -0.3 N.D. N.D. +0.4 N.D.
growth on oleic acid YNB Oleic Acid +0.3 N.D. N.D. -0.3 N.D.
auxotrophy YNB Glucose 75mM Methionine -0.0 N.D. N.D. +0.1 N.D.
ypd buoyancy 40 hrs High Buoyancy +0.1 N.D. N.D. +0.2 N.D.
growth on ricinoleic acid YNB Ricinoleic Acid -0.3 N.D. N.D. +0.7 N.D.
buoyancy on lipid accumulation 40 hrs Median Buoyancy +0.0 N.D. N.D. +0.3 N.D.
auxotrophy YNB Glucose 75mM Arginine +0.0 N.D. N.D. +0.4 N.D.
buoyancy on lipid accumulation 40 hrs Low Buoyancy -0.1 N.D. N.D. +0.5 N.D.
growth on oleic acid YNB Glucose +0.5 N.D. N.D. -0.0 N.D.
growth on ricinoleic acid YNB Glucose -0.1 N.D. N.D. +0.6 N.D.
ypd buoyancy 40 hrs Median Buoyancy +0.3 N.D. N.D. +0.4 N.D.
facs on lipid accumulation Sorted No BODIPY Gate +0.9 N.D. N.D. +0.0 N.D.
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