Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 13230 and 13231 are separated by 217 nucleotides13231 and 13232 are separated by 146 nucleotides13232 and 13233 are separated by 824 nucleotides13233 and 13234 are separated by 320 nucleotides mRNA_4862: 13230 - K00817 hisC histidinol-phosphate aminotransferase, at 1,167,194 to 1,168,610 13230 mRNA_4863: 13231 - KOG0645 WD40 repeat protein, at 1,168,828 to 1,170,396 13231 mRNA_4864: 13232 - KOG0603 Ribosomal protein S6 kinase, at 1,170,543 to 1,172,403 13232 mRNA_4865: 13233 - K12860 CDC5L, CDC5, CEF1 pre-mRNA-splicing factor CDC5/CEF1, at 1,173,228 to 1,176,152 13233 mRNA_4866: 13234 - KOG2881 Predicted membrane protein, at 1,176,473 to 1,177,592 13234
Group Condition mRNA_4862 mRNA_4863 mRNA_4864 mRNA_4865 mRNA_4866
13230 13231 13232 13233 13234
ypd buoyancy 40 hrs No Buoyancy Separation N.D. N.D. -0.5 N.D. -2.5
growth on ypd YPD N.D. N.D. -0.4 N.D. -0.2
ypd buoyancy 40 hrs High Buoyancy N.D. N.D. +0.2 N.D. -0.5
facs on lipid accumulation Sorted Low BODIPY Gate N.D. N.D. +0.4 N.D. -0.7
facs on lipid accumulation Sorted High BODIPY Gate N.D. N.D. +0.2 N.D. -0.4
ypd buoyancy 40 hrs Low Buoyancy N.D. N.D. -0.2 N.D. +0.1
facs on lipid accumulation Sorted No BODIPY Gate N.D. N.D. +0.0 N.D. -0.1
auxotrophy Dropout Complete N.D. N.D. +0.2 N.D. -0.1
growth on oleic acid YNB Glucose N.D. N.D. -0.0 N.D. +0.4
ypd buoyancy 40 hrs Median Buoyancy N.D. N.D. +0.4 N.D. +0.2
auxotrophy YNB Glucose 75mM Methionine N.D. N.D. +0.1 N.D. +0.5
auxotrophy YNB Glucose 75mM Arginine N.D. N.D. +0.4 N.D. +0.3
growth on oleic acid YNB Oleic Acid N.D. N.D. -0.3 N.D. +1.0
auxotrophy YNB Glucose N.D. N.D. +0.4 N.D. +0.5
buoyancy on lipid accumulation 40 hrs Low Buoyancy N.D. N.D. +0.5 N.D. +1.0
buoyancy on lipid accumulation 40 hrs High Buoyancy N.D. N.D. -0.2 N.D. +1.9
growth on ricinoleic acid YNB Glucose N.D. N.D. +0.6 N.D. +1.2
buoyancy on lipid accumulation 40 hrs Median Buoyancy N.D. N.D. +0.3 N.D. +1.5
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation N.D. N.D. +0.5 N.D. +1.6
growth on ricinoleic acid YNB Methylricinoleic Acid N.D. N.D. +0.4 N.D. +2.1
growth on ricinoleic acid YNB Ricinoleic Acid N.D. N.D. +0.7 N.D. +2.7
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