Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 13254 and 13255 are separated by 155 nucleotides13255 and 13256 are separated by 694 nucleotides13256 and 13257 are separated by 87 nucleotides13257 and 13258 are separated by 347 nucleotides mRNA_4886: 13254 - Hypothetical Protein, at 1,220,843 to 1,222,544 13254 mRNA_4887: 13255 - K11365 SGF73 SAGA-associated factor 73, at 1,222,700 to 1,223,919 13255 mRNA_4888: 13256 - K20044 PICALM phosphatidylinositol-binding clathrin assembly protein, at 1,224,614 to 1,227,335 13256 mRNA_4889: 13257 - HMMPfam-Fungal Zn(2)-Cys(6) binuclear cluster domain-PF00172,HMMPfam-Fungal specific transcription factor domain-PF04082,ProSitePatterns-Zn(2)-C6 fungal-type DNA-binding domain signature.-PS00463,ProSiteProfiles-Zn(2)-C6 fungal-type DNA-binding domain profile.-PS50048,SMART-GAL4-like Zn(II)2Cys6 (or C6 zinc) binuclear cluster DNA-binding domain-SM00066,SUPERFAMILY--SSF57701, at 1,227,423 to 1,230,111 13257 mRNA_4890: 13258 - HMMPfam-SH3 domain-PF00018,HMMPfam-Cortical protein marker for cell polarity-PF12768,ProSiteProfiles-Src homology 3 (SH3) domain profile.-PS50002,SMART-Src homology 3 domains-SM00326,SUPERFAMILY--SSF50044,SUPERFAMILY--SSF50965,SUPERFAMILY--SSF69322, at 1,230,459 to 1,234,781 13258
Group Condition mRNA_4886 mRNA_4887 mRNA_4888 mRNA_4889 mRNA_4890
13254 13255 13256 13257 13258
facs on lipid accumulation Sorted High BODIPY Gate -0.0 -0.9 -0.2 -0.6 N.D.
facs on lipid accumulation Sorted Low BODIPY Gate -0.3 +0.7 -0.2 -1.2 N.D.
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation +0.1 -0.3 -0.2 -0.2 N.D.
growth on ypd YPD +0.2 -0.0 -0.3 -0.1 N.D.
auxotrophy Dropout Complete +0.1 +0.6 -0.3 -0.5 N.D.
buoyancy on lipid accumulation 40 hrs Low Buoyancy +0.2 +0.0 +0.0 -0.3 N.D.
growth on ricinoleic acid YNB Glucose +0.1 -0.1 -0.2 +0.0 N.D.
buoyancy on lipid accumulation 40 hrs Median Buoyancy +0.0 +0.0 +0.1 -0.3 N.D.
ypd buoyancy 40 hrs High Buoyancy -0.0 +0.3 -0.4 +0.1 N.D.
ypd buoyancy 40 hrs Median Buoyancy +0.1 -0.2 -0.2 +0.2 N.D.
auxotrophy YNB Glucose 75mM Methionine +0.1 +0.5 -0.3 -0.3 N.D.
facs on lipid accumulation Sorted No BODIPY Gate +0.5 +0.1 +0.2 -0.7 N.D.
buoyancy on lipid accumulation 40 hrs High Buoyancy +0.3 +0.4 -0.2 -0.4 N.D.
auxotrophy YNB Glucose +0.5 +0.1 -0.3 -0.0 N.D.
auxotrophy YNB Glucose 75mM Arginine +0.5 +0.3 -0.2 -0.3 N.D.
ypd buoyancy 40 hrs Low Buoyancy -0.0 +0.7 -0.3 -0.0 N.D.
ypd buoyancy 40 hrs No Buoyancy Separation +0.5 -0.1 -0.6 +0.7 N.D.
growth on ricinoleic acid YNB Methylricinoleic Acid +0.2 +1.0 -0.5 -0.2 N.D.
growth on oleic acid YNB Glucose +0.7 +0.1 -0.1 +0.0 N.D.
growth on ricinoleic acid YNB Ricinoleic Acid +0.5 +0.9 +0.0 -0.4 N.D.
growth on oleic acid YNB Oleic Acid +1.0 +0.6 +0.1 -0.1 N.D.
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