Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 13255 and 13256 are separated by 694 nucleotides13256 and 13257 are separated by 87 nucleotides13257 and 13258 are separated by 347 nucleotides13258 and 13259 are separated by 1217 nucleotides mRNA_4887: 13255 - K11365 SGF73 SAGA-associated factor 73, at 1,222,700 to 1,223,919 13255 mRNA_4888: 13256 - K20044 PICALM phosphatidylinositol-binding clathrin assembly protein, at 1,224,614 to 1,227,335 13256 mRNA_4889: 13257 - HMMPfam-Fungal Zn(2)-Cys(6) binuclear cluster domain-PF00172,HMMPfam-Fungal specific transcription factor domain-PF04082,ProSitePatterns-Zn(2)-C6 fungal-type DNA-binding domain signature.-PS00463,ProSiteProfiles-Zn(2)-C6 fungal-type DNA-binding domain profile.-PS50048,SMART-GAL4-like Zn(II)2Cys6 (or C6 zinc) binuclear cluster DNA-binding domain-SM00066,SUPERFAMILY--SSF57701, at 1,227,423 to 1,230,111 13257 mRNA_4890: 13258 - HMMPfam-SH3 domain-PF00018,HMMPfam-Cortical protein marker for cell polarity-PF12768,ProSiteProfiles-Src homology 3 (SH3) domain profile.-PS50002,SMART-Src homology 3 domains-SM00326,SUPERFAMILY--SSF50044,SUPERFAMILY--SSF50965,SUPERFAMILY--SSF69322, at 1,230,459 to 1,234,781 13258 mRNA_4891: 13259 - KOG3140 Predicted membrane protein, at 1,235,999 to 1,237,638 13259
Group Condition mRNA_4887 mRNA_4888 mRNA_4889 mRNA_4890 mRNA_4891
13255 13256 13257 13258 13259
facs on lipid accumulation Sorted High BODIPY Gate -0.9 -0.2 -0.6 N.D. -0.1
ypd buoyancy 40 hrs High Buoyancy +0.3 -0.4 +0.1 N.D. -1.1
ypd buoyancy 40 hrs No Buoyancy Separation -0.1 -0.6 +0.7 N.D. -1.1
growth on ypd YPD -0.0 -0.3 -0.1 N.D. -0.5
facs on lipid accumulation Sorted No BODIPY Gate +0.1 +0.2 -0.7 N.D. -0.4
ypd buoyancy 40 hrs Median Buoyancy -0.2 -0.2 +0.2 N.D. -0.4
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation -0.3 -0.2 -0.2 N.D. +0.2
facs on lipid accumulation Sorted Low BODIPY Gate +0.7 -0.2 -1.2 N.D. +0.3
ypd buoyancy 40 hrs Low Buoyancy +0.7 -0.3 -0.0 N.D. -0.5
buoyancy on lipid accumulation 40 hrs High Buoyancy +0.4 -0.2 -0.4 N.D. +0.1
growth on ricinoleic acid YNB Glucose -0.1 -0.2 +0.0 N.D. +0.3
buoyancy on lipid accumulation 40 hrs Low Buoyancy +0.0 +0.0 -0.3 N.D. +0.5
growth on ricinoleic acid YNB Ricinoleic Acid +0.9 +0.0 -0.4 N.D. -0.2
buoyancy on lipid accumulation 40 hrs Median Buoyancy +0.0 +0.1 -0.3 N.D. +0.7
growth on ricinoleic acid YNB Methylricinoleic Acid +1.0 -0.5 -0.2 N.D. +0.3
growth on oleic acid YNB Glucose +0.1 -0.1 +0.0 N.D. +0.7
auxotrophy Dropout Complete +0.6 -0.3 -0.5 N.D. +0.9
growth on oleic acid YNB Oleic Acid +0.6 +0.1 -0.1 N.D. +0.3
auxotrophy YNB Glucose +0.1 -0.3 -0.0 N.D. +1.3
auxotrophy YNB Glucose 75mM Arginine +0.3 -0.2 -0.3 N.D. +1.2
auxotrophy YNB Glucose 75mM Methionine +0.5 -0.3 -0.3 N.D. +1.4
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