Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 13256 and 13257 are separated by 87 nucleotides13257 and 13258 are separated by 347 nucleotides13258 and 13259 are separated by 1217 nucleotides13259 and 13260 are separated by 268 nucleotides mRNA_4888: 13256 - K20044 PICALM phosphatidylinositol-binding clathrin assembly protein, at 1,224,614 to 1,227,335 13256 mRNA_4889: 13257 - HMMPfam-Fungal Zn(2)-Cys(6) binuclear cluster domain-PF00172,HMMPfam-Fungal specific transcription factor domain-PF04082,ProSitePatterns-Zn(2)-C6 fungal-type DNA-binding domain signature.-PS00463,ProSiteProfiles-Zn(2)-C6 fungal-type DNA-binding domain profile.-PS50048,SMART-GAL4-like Zn(II)2Cys6 (or C6 zinc) binuclear cluster DNA-binding domain-SM00066,SUPERFAMILY--SSF57701, at 1,227,423 to 1,230,111 13257 mRNA_4890: 13258 - HMMPfam-SH3 domain-PF00018,HMMPfam-Cortical protein marker for cell polarity-PF12768,ProSiteProfiles-Src homology 3 (SH3) domain profile.-PS50002,SMART-Src homology 3 domains-SM00326,SUPERFAMILY--SSF50044,SUPERFAMILY--SSF50965,SUPERFAMILY--SSF69322, at 1,230,459 to 1,234,781 13258 mRNA_4891: 13259 - KOG3140 Predicted membrane protein, at 1,235,999 to 1,237,638 13259 mRNA_4892: 13260 - K00915 IPMK, IPK2 inositol-polyphosphate multikinase, at 1,237,907 to 1,239,157 13260
Group Condition mRNA_4888 mRNA_4889 mRNA_4890 mRNA_4891 mRNA_4892
13256 13257 13258 13259 13260
ypd buoyancy 40 hrs High Buoyancy -0.4 +0.1 N.D. -1.1 N.D.
facs on lipid accumulation Sorted Low BODIPY Gate -0.2 -1.2 N.D. +0.3 N.D.
ypd buoyancy 40 hrs No Buoyancy Separation -0.6 +0.7 N.D. -1.1 N.D.
facs on lipid accumulation Sorted No BODIPY Gate +0.2 -0.7 N.D. -0.4 N.D.
facs on lipid accumulation Sorted High BODIPY Gate -0.2 -0.6 N.D. -0.1 N.D.
growth on ypd YPD -0.3 -0.1 N.D. -0.5 N.D.
ypd buoyancy 40 hrs Low Buoyancy -0.3 -0.0 N.D. -0.5 N.D.
growth on ricinoleic acid YNB Ricinoleic Acid +0.0 -0.4 N.D. -0.2 N.D.
buoyancy on lipid accumulation 40 hrs High Buoyancy -0.2 -0.4 N.D. +0.1 N.D.
ypd buoyancy 40 hrs Median Buoyancy -0.2 +0.2 N.D. -0.4 N.D.
growth on ricinoleic acid YNB Methylricinoleic Acid -0.5 -0.2 N.D. +0.3 N.D.
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation -0.2 -0.2 N.D. +0.2 N.D.
auxotrophy Dropout Complete -0.3 -0.5 N.D. +0.9 N.D.
growth on ricinoleic acid YNB Glucose -0.2 +0.0 N.D. +0.3 N.D.
buoyancy on lipid accumulation 40 hrs Low Buoyancy +0.0 -0.3 N.D. +0.5 N.D.
growth on oleic acid YNB Oleic Acid +0.1 -0.1 N.D. +0.3 N.D.
buoyancy on lipid accumulation 40 hrs Median Buoyancy +0.1 -0.3 N.D. +0.7 N.D.
growth on oleic acid YNB Glucose -0.1 +0.0 N.D. +0.7 N.D.
auxotrophy YNB Glucose 75mM Arginine -0.2 -0.3 N.D. +1.2 N.D.
auxotrophy YNB Glucose -0.3 -0.0 N.D. +1.3 N.D.
auxotrophy YNB Glucose 75mM Methionine -0.3 -0.3 N.D. +1.4 N.D.
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