Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 13258 and 13259 are separated by 1217 nucleotides13259 and 13260 are separated by 268 nucleotides13260 and 13261 are separated by 82 nucleotides13261 and 13262 are separated by 172 nucleotides mRNA_4890: 13258 - HMMPfam-SH3 domain-PF00018,HMMPfam-Cortical protein marker for cell polarity-PF12768,ProSiteProfiles-Src homology 3 (SH3) domain profile.-PS50002,SMART-Src homology 3 domains-SM00326,SUPERFAMILY--SSF50044,SUPERFAMILY--SSF50965,SUPERFAMILY--SSF69322, at 1,230,459 to 1,234,781 13258 mRNA_4891: 13259 - KOG3140 Predicted membrane protein, at 1,235,999 to 1,237,638 13259 mRNA_4892: 13260 - K00915 IPMK, IPK2 inositol-polyphosphate multikinase, at 1,237,907 to 1,239,157 13260 mRNA_4893: 13261 - Hypothetical Protein, at 1,239,240 to 1,240,302 13261 mRNA_4894: 13262 - K11187 PRDX5 peroxiredoxin 5, atypical 2-Cys peroxiredoxin, at 1,240,475 to 1,241,393 13262
Group Condition mRNA_4890 mRNA_4891 mRNA_4892 mRNA_4893 mRNA_4894
13258 13259 13260 13261 13262
ypd buoyancy 40 hrs No Buoyancy Separation N.D. -1.1 N.D. N.D. -0.2
facs on lipid accumulation Sorted No BODIPY Gate N.D. -0.4 N.D. N.D. -0.4
ypd buoyancy 40 hrs High Buoyancy N.D. -1.1 N.D. N.D. +0.5
growth on ricinoleic acid YNB Ricinoleic Acid N.D. -0.2 N.D. N.D. -0.5
growth on ypd YPD N.D. -0.5 N.D. N.D. -0.1
facs on lipid accumulation Sorted High BODIPY Gate N.D. -0.1 N.D. N.D. -0.3
ypd buoyancy 40 hrs Low Buoyancy N.D. -0.5 N.D. N.D. +0.1
ypd buoyancy 40 hrs Median Buoyancy N.D. -0.4 N.D. N.D. +0.3
buoyancy on lipid accumulation 40 hrs High Buoyancy N.D. +0.1 N.D. N.D. -0.2
growth on oleic acid YNB Oleic Acid N.D. +0.3 N.D. N.D. -0.3
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation N.D. +0.2 N.D. N.D. -0.1
growth on ricinoleic acid YNB Methylricinoleic Acid N.D. +0.3 N.D. N.D. -0.1
facs on lipid accumulation Sorted Low BODIPY Gate N.D. +0.3 N.D. N.D. -0.0
growth on ricinoleic acid YNB Glucose N.D. +0.3 N.D. N.D. -0.0
buoyancy on lipid accumulation 40 hrs Low Buoyancy N.D. +0.5 N.D. N.D. -0.1
growth on oleic acid YNB Glucose N.D. +0.7 N.D. N.D. -0.2
buoyancy on lipid accumulation 40 hrs Median Buoyancy N.D. +0.7 N.D. N.D. -0.1
auxotrophy Dropout Complete N.D. +0.9 N.D. N.D. +0.0
auxotrophy YNB Glucose N.D. +1.3 N.D. N.D. -0.1
auxotrophy YNB Glucose 75mM Methionine N.D. +1.4 N.D. N.D. -0.3
auxotrophy YNB Glucose 75mM Arginine N.D. +1.2 N.D. N.D. +0.0
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