Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 13260 and 13261 are separated by 82 nucleotides13261 and 13262 are separated by 172 nucleotides13262 and 13263 are separated by 361 nucleotides13263 and 13264 are separated by 3174 nucleotides mRNA_4892: 13260 - K00915 IPMK, IPK2 inositol-polyphosphate multikinase, at 1,237,907 to 1,239,157 13260 mRNA_4893: 13261 - Hypothetical Protein, at 1,239,240 to 1,240,302 13261 mRNA_4894: 13262 - K11187 PRDX5 peroxiredoxin 5, atypical 2-Cys peroxiredoxin, at 1,240,475 to 1,241,393 13262 mRNA_4895: 13263 - HMMPfam-Etoposide-induced protein 2.4 (EI24)-PF07264, at 1,241,755 to 1,243,025 13263 mRNA_4896: 13264 - KOG0054 Multidrug resistance-associated protein/mitoxantrone resistance protein, ABC superfamily, at 1,246,200 to 1,251,399 13264
Group Condition mRNA_4892 mRNA_4893 mRNA_4894 mRNA_4895 mRNA_4896
13260 13261 13262 13263 13264
facs on lipid accumulation Sorted High BODIPY Gate N.D. N.D. -0.3 -1.1 -0.2
growth on ricinoleic acid YNB Ricinoleic Acid N.D. N.D. -0.5 +0.0 -0.2
growth on oleic acid YNB Glucose N.D. N.D. -0.2 -0.2 -0.2
ypd buoyancy 40 hrs No Buoyancy Separation N.D. N.D. -0.2 -0.5 +0.2
facs on lipid accumulation Sorted No BODIPY Gate N.D. N.D. -0.4 -0.2 -0.0
buoyancy on lipid accumulation 40 hrs High Buoyancy N.D. N.D. -0.2 -0.4 +0.1
auxotrophy Dropout Complete N.D. N.D. +0.0 -0.3 -0.1
growth on oleic acid YNB Oleic Acid N.D. N.D. -0.3 +0.0 -0.1
growth on ricinoleic acid YNB Glucose N.D. N.D. -0.0 -0.1 -0.3
auxotrophy YNB Glucose 75mM Methionine N.D. N.D. -0.3 +0.1 -0.1
growth on ricinoleic acid YNB Methylricinoleic Acid N.D. N.D. -0.1 +0.2 -0.3
facs on lipid accumulation Sorted Low BODIPY Gate N.D. N.D. -0.0 -0.1 -0.0
auxotrophy YNB Glucose N.D. N.D. -0.1 +0.2 -0.2
buoyancy on lipid accumulation 40 hrs Median Buoyancy N.D. N.D. -0.1 +0.0 -0.0
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation N.D. N.D. -0.1 -0.1 +0.2
ypd buoyancy 40 hrs Low Buoyancy N.D. N.D. +0.1 -0.4 +0.3
buoyancy on lipid accumulation 40 hrs Low Buoyancy N.D. N.D. -0.1 -0.0 +0.2
auxotrophy YNB Glucose 75mM Arginine N.D. N.D. +0.0 +0.2 -0.1
growth on ypd YPD N.D. N.D. -0.1 +0.2 +0.3
ypd buoyancy 40 hrs Median Buoyancy N.D. N.D. +0.3 -0.1 +0.2
ypd buoyancy 40 hrs High Buoyancy N.D. N.D. +0.5 -0.0 +0.2
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