Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 13269 and 13270 are separated by 439 nucleotides13270 and 13271 are separated by 118 nucleotides13271 and 13272 are separated by 143 nucleotides13272 and 13273 are separated by 292 nucleotides mRNA_4901: 13269 - K03163 TOP1 DNA topoisomerase I, at 1,259,473 to 1,262,647 13269 mRNA_4902: 13270 - HMMPfam-Fungal Zn(2)-Cys(6) binuclear cluster domain-PF00172,ProSitePatterns-Zn(2)-C6 fungal-type DNA-binding domain signature.-PS00463,ProSiteProfiles-Zn(2)-C6 fungal-type DNA-binding domain profile.-PS50048,SMART-GAL4-like Zn(II)2Cys6 (or C6 zinc) binuclear cluster DNA-binding domain-SM00066,SUPERFAMILY--SSF57701, at 1,263,087 to 1,265,892 13270 mRNA_4903: 13271 - BLAST topoisomerase i [Xanthophyllomyces dendrorhous], at 1,266,011 to 1,266,275 13271 mRNA_4904: 13272 - K00367 narB ferredoxin-nitrate reductase, at 1,266,419 to 1,270,865 13272 mRNA_4905: 13273 - KOG2371 Molybdopterin biosynthesis protein, at 1,271,158 to 1,273,410 13273
Group Condition mRNA_4901 mRNA_4902 mRNA_4903 mRNA_4904 mRNA_4905
13269 13270 13271 13272 13273
growth on ricinoleic acid YNB Methylricinoleic Acid N.D. -0.3 N.D. -0.2 -0.7
growth on ricinoleic acid YNB Ricinoleic Acid N.D. -0.4 N.D. -0.4 -0.3
growth on ricinoleic acid YNB Glucose N.D. -0.1 N.D. -0.3 -0.3
growth on oleic acid YNB Oleic Acid N.D. -0.2 N.D. -0.4 -0.1
auxotrophy Dropout Complete N.D. -0.0 N.D. -0.3 -0.3
facs on lipid accumulation Sorted No BODIPY Gate N.D. +0.1 N.D. -0.1 -0.4
auxotrophy YNB Glucose N.D. -0.1 N.D. -0.2 -0.1
auxotrophy YNB Glucose 75mM Methionine N.D. -0.1 N.D. -0.1 -0.2
facs on lipid accumulation Sorted Low BODIPY Gate N.D. -0.3 N.D. -0.1 +0.1
growth on oleic acid YNB Glucose N.D. -0.1 N.D. -0.2 -0.1
ypd buoyancy 40 hrs High Buoyancy N.D. -0.1 N.D. -0.0 -0.2
facs on lipid accumulation Sorted High BODIPY Gate N.D. -0.2 N.D. +0.1 -0.2
growth on ypd YPD N.D. +0.1 N.D. -0.1 -0.2
ypd buoyancy 40 hrs Low Buoyancy N.D. -0.2 N.D. +0.1 -0.1
ypd buoyancy 40 hrs Median Buoyancy N.D. -0.1 N.D. +0.1 -0.1
buoyancy on lipid accumulation 40 hrs Median Buoyancy N.D. +0.1 N.D. +0.0 -0.2
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation N.D. +0.1 N.D. -0.1 -0.0
auxotrophy YNB Glucose 75mM Arginine N.D. +0.2 N.D. -0.1 +0.0
buoyancy on lipid accumulation 40 hrs High Buoyancy N.D. +0.0 N.D. +0.1 +0.1
buoyancy on lipid accumulation 40 hrs Low Buoyancy N.D. +0.3 N.D. +0.1 -0.1
ypd buoyancy 40 hrs No Buoyancy Separation N.D. +0.0 N.D. +0.5 +0.2
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