Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 13273 and 13274 are separated by 58 nucleotides13274 and 13275 are separated by 288 nucleotides13275 and 13276 are separated by 281 nucleotides13276 and 13277 are separated by 495 nucleotides mRNA_4905: 13273 - KOG2371 Molybdopterin biosynthesis protein, at 1,271,158 to 1,273,410 13273 mRNA_4906: 13274 - K03540 RPR2, RPP21 ribonuclease P protein subunit RPR2, at 1,273,469 to 1,274,303 13274 mRNA_4907: 13275 - HMMPfam-Cytochrome c oxidase subunit VIIc-PF02935,SUPERFAMILY--SSF81427, at 1,274,592 to 1,274,946 13275 mRNA_4908: 13276 - K15163 SRB8, MED12 mediator of RNA polymerase II transcription subunit 12, fungi type, at 1,275,228 to 1,281,484 13276 mRNA_4909: 13277 - KOG1339 Aspartyl protease, at 1,281,980 to 1,284,451 13277
Group Condition mRNA_4905 mRNA_4906 mRNA_4907 mRNA_4908 mRNA_4909
13273 13274 13275 13276 13277
facs on lipid accumulation Sorted No BODIPY Gate -0.4 N.D. N.D. -1.2 -0.1
growth on ricinoleic acid YNB Ricinoleic Acid -0.3 N.D. N.D. -1.3 -0.1
facs on lipid accumulation Sorted High BODIPY Gate -0.2 N.D. N.D. -1.3 -0.2
growth on ricinoleic acid YNB Methylricinoleic Acid -0.7 N.D. N.D. -0.2 +0.1
facs on lipid accumulation Sorted Low BODIPY Gate +0.1 N.D. N.D. -1.0 +0.0
growth on ypd YPD -0.2 N.D. N.D. -0.0 -0.2
buoyancy on lipid accumulation 40 hrs Median Buoyancy -0.2 N.D. N.D. -0.3 +0.2
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation -0.0 N.D. N.D. -0.2 +0.1
ypd buoyancy 40 hrs High Buoyancy -0.2 N.D. N.D. -0.0 +0.0
growth on ricinoleic acid YNB Glucose -0.3 N.D. N.D. -0.0 +0.2
growth on oleic acid YNB Oleic Acid -0.1 N.D. N.D. +0.1 -0.1
auxotrophy YNB Glucose 75mM Methionine -0.2 N.D. N.D. +0.2 -0.1
buoyancy on lipid accumulation 40 hrs Low Buoyancy -0.1 N.D. N.D. -0.1 +0.1
auxotrophy Dropout Complete -0.3 N.D. N.D. +0.1 +0.1
ypd buoyancy 40 hrs Low Buoyancy -0.1 N.D. N.D. +0.1 -0.0
ypd buoyancy 40 hrs Median Buoyancy -0.1 N.D. N.D. +0.1 -0.0
auxotrophy YNB Glucose -0.1 N.D. N.D. +0.0 +0.1
ypd buoyancy 40 hrs No Buoyancy Separation +0.2 N.D. N.D. +0.1 -0.0
growth on oleic acid YNB Glucose -0.1 N.D. N.D. +0.2 +0.1
auxotrophy YNB Glucose 75mM Arginine +0.0 N.D. N.D. +0.1 +0.2
buoyancy on lipid accumulation 40 hrs High Buoyancy +0.1 N.D. N.D. +0.0 +0.2
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