Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 13286 and 13287 are separated by 371 nucleotides13287 and 13288 are separated by 749 nucleotides13288 and 13289 are separated by 507 nucleotides13289 and 13290 are separated by 426 nucleotides mRNA_4918: 13286 - K00327 POR NADPH-ferrihemoprotein reductase, at 1,301,250 to 1,303,860 13286 mRNA_4919: 13287 - K01768 E4.6.1.1 adenylate cyclase, at 1,304,232 to 1,310,691 13287 mRNA_4920: 13288 - K01807 rpiA ribose 5-phosphate isomerase A, at 1,311,441 to 1,312,877 13288 mRNA_4921: 13289 - K12156 CYP83A1 cytochrome P450, family 83, subfamily A, polypeptide 1, at 1,313,385 to 1,315,387 13289 mRNA_4922: 13290 - K18670 YAK1 dual specificity protein kinase YAK1, at 1,315,814 to 1,320,351 13290
Group Condition mRNA_4918 mRNA_4919 mRNA_4920 mRNA_4921 mRNA_4922
13286 13287 13288 13289 13290
facs on lipid accumulation Sorted High BODIPY Gate N.D. +0.3 N.D. +0.4 -1.7
ypd buoyancy 40 hrs No Buoyancy Separation N.D. -0.5 N.D. +0.0 -0.3
buoyancy on lipid accumulation 40 hrs Low Buoyancy N.D. -0.4 N.D. -0.7 +0.9
facs on lipid accumulation Sorted Low BODIPY Gate N.D. +0.4 N.D. +0.2 -0.8
facs on lipid accumulation Sorted No BODIPY Gate N.D. +0.1 N.D. +0.0 -0.2
ypd buoyancy 40 hrs Median Buoyancy N.D. -0.2 N.D. -0.0 +0.1
buoyancy on lipid accumulation 40 hrs Median Buoyancy N.D. +0.6 N.D. -0.2 -0.3
ypd buoyancy 40 hrs High Buoyancy N.D. -0.3 N.D. +0.3 +0.0
ypd buoyancy 40 hrs Low Buoyancy N.D. +0.1 N.D. -0.2 +0.5
buoyancy on lipid accumulation 40 hrs High Buoyancy N.D. +0.7 N.D. -0.0 -0.2
growth on ypd YPD N.D. +0.1 N.D. +0.4 -0.0
auxotrophy YNB Glucose 75mM Methionine N.D. +0.7 N.D. +0.0 -0.1
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation N.D. +0.3 N.D. +0.1 +0.2
growth on oleic acid YNB Oleic Acid N.D. +0.7 N.D. +0.2 -0.3
auxotrophy YNB Glucose 75mM Arginine N.D. +0.8 N.D. -0.0 -0.1
growth on oleic acid YNB Glucose N.D. +0.4 N.D. +0.4 -0.1
auxotrophy YNB Glucose N.D. +0.7 N.D. +0.2 -0.1
growth on ricinoleic acid YNB Glucose N.D. +0.7 N.D. +0.0 +0.1
auxotrophy Dropout Complete N.D. +0.7 N.D. +0.2 +0.1
growth on ricinoleic acid YNB Ricinoleic Acid N.D. +1.2 N.D. +0.5 -0.4
growth on ricinoleic acid YNB Methylricinoleic Acid N.D. +1.0 N.D. +0.2 +0.2
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